Biglycan Antibody

Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NB100-55407] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NB100-55407] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Biglycan Antibody Summary

• Description: Novus Biologicals Goat Biglycan Antibody (NB100-55407) is a polyclonal antibody validated for use in IHC, ELISA and ICC/IF. Anti-Biglycan Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Biglycan Antibody is made to a peptide with sequence C-DRLAIQFGNYKK corresponding to C-Terminus according to NP_001702.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BGN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Peptide ELISA Detection limit 1:2000
• Application Notes: IHC successful usage on human achilles tendons is reported in scientific literature (PMID: 23001016). Use in ELISA reported in scientific literature (PMID:26694746)
• Theoretical MW: 41.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Biglycan Overexpression Lysate

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 22427827).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Biglycan Antibody

• BGN
• Biglycan
• Bone/cartilage proteoglycan I
• bone/cartilage proteoglycan-I
• dermatan sulphate proteoglycan I
• DSPG1
• PGI
• PG-S1
• SLRR1A
• SLRR1Abiglycan proteoglycan
• small leucine-rich protein 1A

Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Biglycan Antibody (NB100-55407)(4)

Publications using NB100-55407

• Reine TM, Vuong TT, Rutkovskiy A et al. Serglycin in Quiescent and Proliferating Primary Endothelial Cells. PLoS One 2015-01-01 [PMID: 26694746] (ELISA)
• Burssens A, Forsyth R, Bongaerts W et al. Arguments for an increasing differentiation towards fibrocartilaginous components in midportion Achilles tendinopathy. Knee Surg Sports Traumatol Arthrosc 2012-09-23 [PMID: 23001016]
• Antipova O, Orgel J. Non-Enzymatic Decomposition of Collagen Fibers by a Biglycan Antibody and a Plausible Mechanism for Rheumatoid Arthritis PLoS ONE 2012-01-01 [PMID: 22427827] (Human, Bovine, Rat)
• Chen WB, Lenschow W, Tiede K et al. Smad4/DPC4-dependent regulation of biglycan gene expression by transforming growth factor-beta in pancreatic tumor cells. J Biol Chem 2002-09-27 [PMID: 12140283]

Bioinformatics

• Gene Symbol: BGN
• Entrez:
  • 633(Human)
  • 12111(Mouse)
  • 25181(Rat)
• Uniprot:
  • P21810()