Biglycan Antibody

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Western Blot: Biglycan Antibody [NB100-55407] - (0.1ug/ml) staining of Rat Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Biglycan Antibody Summary

Immunogen
Peptide with sequence C-DRLAIQFGNYKK corresponding to C-Terminus according to NP_001702.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BGN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 37 kDa band observed in rat skin lysates (calculated MW of 41.7 kDa band according to human NP_001702.1 and rat NP_058783.1). IHC successful usage on human achilles tendons is reported in scientific literature (PMID: 23001016).
Theoretical MW
41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Biglycan Lysate (NBL1-07973)
Publications
Read Publications using
NB100-55407 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Biglycan Antibody

  • BGN
  • Biglycan
  • Bone/cartilage proteoglycan I
  • bone/cartilage proteoglycan-I
  • dermatan sulphate proteoglycan I
  • DSPG1
  • PGI
  • PG-S1
  • SLRR1A
  • SLRR1Abiglycan proteoglycan
  • small leucine-rich protein 1A

Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Biglycan Antibody (NB100-55407)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BGN
Entrez
Uniprot