BID Antibody

Western Blot: BID Antibody [NB100-2206] - Analysis of BID expression in 3T3 cell lysate.

Product Discontinued

BID Antibody Summary

• Immunogen: Synthetic peptide surrounding amino acid 45 of mouse Bid.
• Localization: Cytoplasmic
• Specificity: This detects both full length (22 kDa) and weakly reacts wtih cleaved fragment (15 kDa) of mouse Bid.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BID
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 20 ug/mL
  • Immunoprecipitation 20 ug/mL
  • Western Blot 0.5 - 5 ug/mL

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for BID Antibody

• apoptic death agonist
• BH3 interacting domain death agonist
• BH3-interacting domain death agonist
• BID isoform ES(1b)
• BID isoform L(2)
• BID isoform Si6
• BID
• desmocollin type 4
• FP497
• Human BID coding sequence
• MGC15319
• MGC42355
• p22 BID
• tbid

Background

Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BID
• Entrez:
  • 12122(Mouse)
• Uniprot:
  • P70444()