BID Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µg/mL anti-Fas Antibody for 6 hours and WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BID Polyclonal Antibody (Catalog # AF846), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BID at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Product Discontinued

BID Antibody Summary

• Immunogen: KLH-coupled human BID synthetic peptide
  RRELDALGHELPVLAPQWEC
• Specificity: Detects human BID in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse BID is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BID
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BID Antibody

• apoptic death agonist
• BH3 interacting domain death agonist
• BH3-interacting domain death agonist
• BID isoform ES(1b)
• BID isoform L(2)
• BID isoform Si6
• BID
• desmocollin type 4
• FP497
• Human BID coding sequence
• MGC15319
• MGC42355
• p22 BID
• tbid

Background

Bid is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF, Bid is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of Bid by caspase-8 generates a new N-terminous that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria (4). Bid may then interact withanother pro-apoptotic Bcl-2 family member Bak (5). Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A (9-13) amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the Bid interaction with other Bcl-2 family members. Bid is neutralized by binding to the anti‑apoptotic member Bcl-xL.

 

1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
2. Luo, X., et al. (1998) Cell 94:481.
3. Li, H. et al. (1998) Cell 94:491.
4. Zha, J. et al. (2000) Science 290:1761.
5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BID Antibody (AF846)(6)

Publications using AF846

• A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells PLoS ONE, 2019-04-04;14(4):e0214847. 2019-04-04 [PMID: 30947287] (Western Blot, Human)
• A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment Cell Death Differ., 2017-11-03;0(0):. 2017-11-03 [PMID: 29099485]
• Caspase-2-mediated cell death is required for deleting aneuploid cells Oncogene, 2016-12-19;0(0):. 2016-12-19 [PMID: 27991927] (Western Blot, Human)
• Ikner A, Ashkenazi A TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8. J. Biol. Chem., 2011-04-27;286(24):21546-54. 2011-04-27 [PMID: 21525013] (Western Blot, Human)
• Shah N, Asch RJ, Lysholm AS, Lebien TW Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor. Blood, 2004-07-08;104(9):2873-8. 2004-07-08 [PMID: 15242874] (Western Blot, Human)
• He Q, Lee DI, Rong R, Yu M, Luo X, Klein M, El-Deiry WS, Huang Y, Hussain A, Sheikh MS Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway. Oncogene, 2002-04-18;21(17):2623-33. 2002-04-18 [PMID: 11965535] (Western Blot, Human)

Review for BID Antibody (AF846) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24030153
• Species: Human

Bioinformatics

• Gene Symbol: BID