Recombinant Human Betacellulin/BTC Protein

Product Discontinued

Recombinant Human Betacellulin/BTC Protein Summary

• Description: Recombinant bioactive protein containing 80 amino acids for Human Betacellulin/BTC
  
  

  Source:E. coli
  Amino Acid Sequence:(P35070)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 0.05 ng/ml; corresponding to a Specific Activity of > 2.0 x107IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BTC
• Purity: >97%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized after dialysis against 20 mM phosphate buffer (pH 7.4).
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile distilled H2O to > 100 ug/ml. This solution can then be diluted into other aqueous buffers.

Alternate Names for Recombinant Human Betacellulin/BTC Protein

• Betacellulin
• BTC
• probetacellulin

Background

Human Betacellulin (BTC) is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the egf receptor and other related receptors. Human Recombinant BTC produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. BTC was purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BTC