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beta Tubulin Antibody (4E4)

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Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047] - Analysis of equal amounts of cell line and whole brain lysates using mouse mAb to Beta-tubulin NBP2-50047, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] NIH-3T3, [7] C6 cells, [8] rat brain, and [9] mouse brain. Strong band at 50 kDa corresponds to the Beta-tubulin proteins.
Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047] - Analysis of equal amounts of cell line and whole brain lysates using mouse mAb to Beta-tubulin NBP2-50047, dilution 1:5,000 in green: [1] protein standard (red), ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E4) [NBP2-50047] - HeLa cells stained with NBP2-50047 (green) and chicken antibody to Lamin A+C (NBP2-25152, red) and DNA in blue. NBP2-50047 reveals ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-50047AF405
Alexa Fluor 488
NBP2-50047AF488
Alexa Fluor 647
NBP2-50047AF647
Alexa Fluor 700
NBP2-50047AF700
Biotin
NBP2-50047B
DyLight 350
NBP2-50047UV
DyLight 405
NBP2-50047V
DyLight 488
NBP2-50047G
DyLight 550
NBP2-50047R
DyLight 650
NBP2-50047C
DyLight 680
NBP2-50047FR
DyLight 755
NBP2-50047IR
FITC
NBP2-50047F
HRP
NBP2-50047H
Janelia Fluor 549
NBP2-50047JF549
Janelia Fluor 646
NBP2-50047JF646
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beta Tubulin Antibody (4E4) Summary

Immunogen
Pig brain beta Tubulin. [UniProt# Q767L7]
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000-1:5000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta Tubulin Antibody (4E4)

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Galanin Antibody [NBP2-33582] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining in human appendix and cerebral cortex tissues using anti-GAL antibody. Corresponding GAL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human anterior pituitary gland shows strong cytoplasmic positivity in subsets of endocrine cells.
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(1 Publication)
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GAPDH Antibody
(1D4)
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Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
(17 Reviews)
(282 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Op, Pm, Rb, Sh, Ze
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Western Blot: Complement C7 Antibody [H00000730-D01P] - Analysis of C7 expression in mouse kidney.
Western Blot: Complement C7 Antibody [H00000730-D01P] - Analysis of C7 expression in transfected 293T cell line by C7 polyclonal antibody.Lane 1: C7 transfected lysate(93.50 KDa).Lane 2: Non-transfected lysate.
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beta-III Tubulin
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Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained forbeta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. Image from verified customer review.
Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
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(33 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Galanin R1/GALR1
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Immunohistochemistry-Paraffin: Galanin R1/GALR1 Antibody [NLS6168] - Anti-Galanin Receptor 1 / GALR1 antibody IHC of human heart, myocardium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 100 ug/ml.
Immunohistochemistry-Paraffin: Galanin R1/GALR1 Antibody [NLS6168] - Analysis of anti-Galanin Receptor 1 / GALR1 antibody with human brain, hypothalamus arcuate nucleus at dilution 100 ug/ml.
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Galanin Antibody
NBP2-33582
Immunocytochemistry/Immunofluorescence: Galanin Antibody [NBP2-33582] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining in human appendix and cerebral cortex tissues using anti-GAL antibody. Corresponding GAL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human anterior pituitary gland shows strong cytoplasmic positivity in subsets of endocrine cells.
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Antibody
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Neuropeptide Y Antibody
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Species: Hu, Mu, Rt, Rb, Xp, Ze
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Tubulin Beta 2C
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Western Blot: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Analysis of beta II Tubulin on different lysates using anti-beta II Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Mouse kidney
Immunocytochemistry/Immunofluorescence: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Staining beta II Tubulin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: F-Actin Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.
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Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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RPL10 Antibody
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Western Blot: RPL10 Antibody [NBP1-84037] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: RPL10 Antibody [NBP1-84037] - Analysis in human cell line HeLa.
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NOV/CCN3 Antibody
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NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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DNMBP Antibody
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Western Blot: DNMBP Antibody [H00023268-B01P] - Analysis of DNMBP expression in HeLa.
Immunocytochemistry/Immunofluorescence: DNMBP Antibody [H00023268-B01P] - Analysis of purified antibody to DNMBP on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: DNMBP Antibody [H00023268-B01P] - Analysis of DNMBP expression in transfected 293T cell line by DNMBP polyclonal antibody. Lane 1: DNMBP transfected lysate(90.53 KDa). Lane 2: Non-transfected lysate.
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beta-Actin
Antibody (AC-15)
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Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
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Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
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Midkine Antibody
[Unconjugated]
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Midkine was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Midkine at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Midkine at approximately 26 kDa (as indicated) using 100 µg/mL of Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
(4 Publications)
Species: Hu
Applications: WB, Simple Western, IHC

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NBP2-50047). (Showing 1 - 1 of 1 FAQ).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-50047AF405
Alexa Fluor 488 NBP2-50047AF488
Alexa Fluor 647 NBP2-50047AF647
Alexa Fluor 700 NBP2-50047AF700
Biotin NBP2-50047B
DyLight 350 NBP2-50047UV
DyLight 405 NBP2-50047V
DyLight 488 NBP2-50047G
DyLight 550 NBP2-50047R
DyLight 650 NBP2-50047C
DyLight 680 NBP2-50047FR
DyLight 755 NBP2-50047IR
FITC NBP2-50047F
HRP NBP2-50047H
Janelia Fluor 549 NBP2-50047JF549
Janelia Fluor 646 NBP2-50047JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol TUBB
Uniprot