beta-TrCP1/BTRC Antibody - BSA Free

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP1 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: beta-TrCP1/BTRC Antibody [NBP1-33301] - Paraffin-embedded rat fore brain. beta-TrCP antibody [N1C1] dilution: 1:250.

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP1/BTRC antibody [N1C1] (NBP1-33301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt, Mu, Bv, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

beta-TrCP1/BTRC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit beta-TrCP1/BTRC Antibody - BSA Free (NBP1-33301) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human beta-TrCP1/BTRC. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Mouse (99%), Bovine (99%), Xenopus (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTRC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for beta-TrCP1/BTRC Antibody - BSA Free

• beta-transducin repeat containing
• betaTrCP
• BETA-TRCP
• betaTrCP1
• beta-TrCP1
• BTRC
• BTRCP
• bTrCP1
• E3RSIkappaB
• F-box and WD repeats protein beta-TrCP
• F-box/WD repeat-containing protein 1A
• FBW1A
• FBXW1
• FBXW1A
• FBXW1ABTRCP
• FWD1
• MGC4643
• pIkappaBalpha-E3 receptor subunit

Background

betaTrCP encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BTRC