beta Sarcoglycan Overexpression Lysate (Native)


Western Blot: beta Sarcoglycan Overexpression Lysate (Adult Normal) [NBL1-15903] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for beta Sarcoglycan.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

beta Sarcoglycan Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC204699 and based on accession number NM_000232. The protein contains a C-terminal DDK tag.
Homo sapiens sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) (SGCB), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
34.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for beta Sarcoglycan

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for beta Sarcoglycan Overexpression Lysate (Native)

  • 43 kDa dystrophin-associated glycoprotein
  • 43DAG
  • A3b
  • A3bbeta-SG
  • beta Sarcoglycan
  • beta-Sarcoglycan
  • beta-sarcoglycan(43kD dystrophin-associated glycoprotein)
  • Beta-SG
  • LGMD2E
  • limb girdle muscular dystrophy 2E (non-linked families)
  • Sarcoglycan beta
  • sarcoglycan, beta (43kD dystrophin-associated glycoprotein)
  • sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
  • SGC
  • SGCB


This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with limb-girdle muscular dystrophy. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
Applications: WB, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, IA, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB

Publications for beta Sarcoglycan Lysate (NBL1-15903) (0)

There are no publications for beta Sarcoglycan Lysate (NBL1-15903).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta Sarcoglycan Lysate (NBL1-15903) (0)

There are no reviews for beta Sarcoglycan Lysate (NBL1-15903). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta Sarcoglycan Lysate (NBL1-15903) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional beta Sarcoglycan Products

Bioinformatics Tool for beta Sarcoglycan Lysate (NBL1-15903)

Discover related pathways, diseases and genes to beta Sarcoglycan Lysate (NBL1-15903). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta Sarcoglycan Lysate (NBL1-15903)

Discover more about diseases related to beta Sarcoglycan Lysate (NBL1-15903).

Pathways for beta Sarcoglycan Lysate (NBL1-15903)

View related products by pathway.

PTMs for beta Sarcoglycan Lysate (NBL1-15903)

Learn more about PTMs related to beta Sarcoglycan Lysate (NBL1-15903).

Blogs on beta Sarcoglycan

There are no specific blogs for beta Sarcoglycan, but you can read our latest blog posts.

Customers Who Bought This Also Bought

iNOS Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our beta Sarcoglycan Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol SGCB