beta Sarcoglycan Antibody

Immunocytochemistry/Immunofluorescence: beta Sarcoglycan Antibody [NBP2-54977] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Frozen: beta Sarcoglycan Antibody [NBP2-54977] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.

Product Discontinued

beta Sarcoglycan Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GPNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGNEGVFIMGKT
• Predicted Species: Mouse (99%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGCB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry-Frozen
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Beta Sarcoglycan Antibody validated for IHC-Fr from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for beta Sarcoglycan Antibody

• 43 kDa dystrophin-associated glycoprotein
• 43DAG
• A3b
• A3bbeta-SG
• beta Sarcoglycan
• beta-Sarcoglycan
• beta-sarcoglycan(43kD dystrophin-associated glycoprotein)
• Beta-SG
• LGMD2E
• limb girdle muscular dystrophy 2E (non-linked families)
• Sarcoglycan beta
• sarcoglycan, beta (43kD dystrophin-associated glycoprotein)
• sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
• SGC
• SGCB

Background

The dystrophin-glycoprotein complex (DGC) is a multisubunit protein complex that spans the sarcolemma and provides structural linkage between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. There are 3 main subcomplexes of the DGC: the cytoplasmic proteins dystrophin (DMD; MIM 300377) and syntrophin (SNTA1; MIM 601017), the alpha- and beta-dystroglycans (see MIM 128239), and the sarcoglycans (see, e.g., SGCA; MIM 600119) (Crosbie et al., 2000 [PubMed 10942431]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Sarcoglycan Antibody (NBP2-54977)(1)

Publication using NBP2-54977

• Siemionow M, Cwykiel J, Heydemann A et al. Dystrophin Expressing Chimeric (DEC) Human Cells Provide a Potential Therapy for Duchenne Muscular Dystrophy Stem Cell Rev 2018-03-15 [PMID: 29546607] (ICC/IF, Human)

Review for beta Sarcoglycan Antibody (NBP2-54977) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Margherita verardo IHC-Fr Human 10/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Skeletal muscle tissue
• Species: Human
• Lot: R81984

Bioinformatics

• Gene Symbol: SGCB