beta-Glucuronidase/GUSB Antibody (105)

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Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications WB, ELISA, IHC, IHC-Fr
Clone
105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-Glucuronidase/GUSB Antibody (105) Summary

Immunogen
The antibody reacts with the Beta-Glucuronidase antigen.
Specificity
This antibody reacts with an non-active center epitope on human placental and liver beta-Glucuronidase. Does not cross react with rat beta-Glucuronidase.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GUSB
Purity
Tissue culture supernatant
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This antibody is useful for staining of human Beta-Glucuronidase in ELISA, Western blotting and frozen sections. The antibody can also be used for affinity purification of human Beta-Glucuronidase. The antibody apparently reacts with the native enzyme only (the advised working dilution is 1:10.)

Reactivity Notes

Human,Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for beta-Glucuronidase/GUSB Antibody (105)

  • beta-D-glucuronidase
  • Beta-G1
  • beta-glucuronidase
  • BG
  • EC 3.2.1.31
  • FLJ39445
  • glucuronidase, beta
  • GUSB
  • MPS7

Background

The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GUSB
Entrez