beta-Glucuronidase/GUSB Antibody (105)

Product Discontinued

beta-Glucuronidase/GUSB Antibody (105) Summary

• Immunogen: The antibody reacts with the Beta-Glucuronidase antigen.
• Specificity: This antibody reacts with an non-active center epitope on human placental and liver beta-Glucuronidase. Does not cross react with rat beta-Glucuronidase.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GUSB
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is useful for staining of human Beta-Glucuronidase in ELISA, Western blotting and frozen sections. The antibody can also be used for affinity purification of human Beta-Glucuronidase. The antibody apparently reacts with the native enzyme only (the advised working dilution is 1:10.)

Reactivity Notes

Human,Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for beta-Glucuronidase/GUSB Antibody (105)

• beta-D-glucuronidase
• Beta-G1
• beta-glucuronidase
• BG
• EC 3.2.1.31
• FLJ39445
• glucuronidase, beta
• GUSB
• MPS7

Background

The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GUSB