beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - Analysis of GLB1 expression in transfected 293T cell line by GLB1 monoclonal antibody (M01), clone 6E7.Lane 1: GLB1 transfected lysate(76.1 KDa).Lane 2: Non-transfected lysate.

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - GLB1 monoclonal antibody (M01), clone 6E7 Analysis of GLB1 expression in HeLa.

Immunoprecipitation: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - Analysis of GLB1 transfected lysate using anti-GLB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLB1 MaxPab rabbit polyclonal antibody.

ELISA: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - Detection limit for recombinant GST tagged GLB1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 6E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GLB1 (NP_000395, 578 a.a. ~ 677 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV
• Specificity: GLB1 - galactosidase, beta 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

• Acid beta-galactosidase
• beta-galactosidase
• betaGalactosidase1
• beta-Galactosidase-1
• EBP
• EC 3.2.1.23
• elastin receptor 1 (67kD)
• Elastin receptor 1
• elastin receptor 1, 67kDa
• ELNR1
• galactosidase, beta 1
• GLB1
• Lactase
• MPS4B

Background

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLB1
• Entrez:
  • 2720(Human)
• OMIM:
  • 230500(Human)
• Uniprot:
  • NP_000395(Human)