Recombinant Mouse beta-Defensin 3 Protein

SDS-Page: Mouse beta-Defensin 3 Protein [NBP2-35146]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse beta-Defensin 3 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 41 amino acid residues corresponding to beta-Defensin 3 Source: E. coli

  Uniprot ID: Q9WTL0

  Amino Acid Sequence: KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K

• Details of Functionality: Defensin beta 3 Protein is fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 20 ug/ml, corresponding to a specific activity of > 50 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DEFB103B
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of beta-Defensin 3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 4.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse beta-Defensin 3 Protein

• BD14
• BD3
• BD-3
• beta-defensin 103
• betaDefensin 3
• beta-Defensin 3
• DEFB103
• DEFB103A
• DEFB103B
• DEFB3
• DEFB-3
• DEFB3DEFB103A
• Defensin, beta 103
• defensin, beta 103B
• Defensin-like protein
• HBD-3
• HBD3HBP3

Background

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 k Da proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. beta-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human beta-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because beta-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca 2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DEFB103B
• Uniprot:
  • Q9WTL0()
  • Q9WTL0()
  • Q9WTL0()