beta 2-Microglobulin Antibody (SU35-05)

Western Blot: beta 2-Microglobulin Antibody (SU35-05) [NBP2-67474] - Analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: U937

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (SU35-05) [NBP2-67474] - Staining beta 2 Microglobulin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (SU35-05) [NBP2-67474] - Staining beta 2 Microglobulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

beta 2-Microglobulin Antibody (SU35-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human beta 2-Microglobulin (SwissProt: P61769 Human; SwissProt: P01887 Mouse; SwissProt: P07151 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: B2M
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for beta 2-Microglobulin Antibody (SU35-05)

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M