| Reactivity | Hu, Pm(-)Species Glossary |
| Applications | Flow, ICC/IF |
| Clone | SPM374 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 650 |
| Immunogen | Human PBL s from a T-cell acute lymphoblastic leukemia (T-ALL) patient. |
| Localization | Cell Surface and Extracellular (Secreted) |
| Marker | Renal Failure & Tumor Marker |
| Specificity | Recognizes a protein of 12kDa, identified as b2-microglobulin. Beta-2-microglobulin non-covalently associates with the 44kDa a chain to form the HLA Class I antigen complex. Human beta-2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This MAb is specific to human b2-microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. The detection of beta2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | B2M |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-47701AF350 | |
| Alexa Fluor 405 | NBP2-47701AF405 | |
| Alexa Fluor 488 | NBP2-47701AF488 | |
| Alexa Fluor 532 | NBP2-47701AF532 | |
| Alexa Fluor 594 | NBP2-47701AF594 | |
| Alexa Fluor 647 | NBP2-47701AF647 | |
| Alexa Fluor 700 | NBP2-47701AF700 | |
| Alexa Fluor 750 | NBP2-47701AF750 | |
| Allophycocyanin | NBP2-47701APC | |
| Allophycocyanin/Cy7 | NBP2-47701APCCY7 | |
| Biotin | NBP2-47701B | |
| Biotin | NBP2-47701B | |
| DyLight 350 | NBP2-47701UV | |
| DyLight 405 | NBP2-47701V | |
| DyLight 488 | NBP2-47701G | |
| DyLight 550 | NBP2-47701R | |
| DyLight 594 | NBP2-47701DL594 | |
| DyLight 650 | NBP2-47701C | |
| DyLight 680 | NBP2-47701FR | |
| DyLight 755 | NBP2-47701IR | |
| FITC | NBP2-47701F | |
| Janelia Fluor 549 | NBP2-47701JF549 | |
| Janelia Fluor 646 | NBP2-47701JF646 | |
| PE | NBP2-47701PE | |
| PE/Atto594 | NBP2-47701PEATT594 | |
| PE/Cy5.5 | NBP2-47701PECY55 | |
| PE/Cy7 | NBP2-47701PECY7 | |
| PerCP | NBP2-47701PCP |
Diseases for beta 2-Microglobulin Antibody (NBP2-47701C)Discover more about diseases related to beta 2-Microglobulin Antibody (NBP2-47701C).
| Pathways for beta 2-Microglobulin Antibody (NBP2-47701C)View related products by pathway.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | B2M |
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.](http://images.novusbio.com/images2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.](http://images.novusbio.com/images2/GAPDH-Antibody-(1D4)-Immunocytochemistry-Immunofluorescence-NB300-221-img0009.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.](http://images.novusbio.com/images2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0008.jpg)
![Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.](http://images.novusbio.com/images2/beta-Actin-Antibody-(AC-15)-Western-Blot-NB600-501-img0023.jpg)
![Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/images2/beta-Actin-Antibody-(AC-15)-Simple-Western-NB600-501-img0028.jpg)
![Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.](http://images.novusbio.com/images2/beta-Actin-Antibody-(AC-15)-Western-Blot-NB600-501-img0026.jpg)
![Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (http://neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Actin-Gamma-1-Antibody-Western-Blot-NB600-533-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/images2/Actin-Gamma-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-533-img0003.jpg)
![Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/Actin-Gamma-1-Antibody-Immunohistochemistry-NB600-533-img0008.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/HPRT-Antibody-Western-Blot-NBP1-33527-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/images2/HPRT Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33527-img0002.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.](http://images.novusbio.com/images2/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0013.jpg)
![Western Blot: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - Whole cell extracts (40 ug) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against TBP diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/images2/TATA-binding-protein-TBP-Antibody-(C.15200002)-Western-Blot-NBP2-59208-img0001.jpg)
![Chromatin Immunoprecipitation Sequencing: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - IP'd DNA was analyzed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (A). The IP'd DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.](http://images.novusbio.com/images2/TATA-binding-protein-TBP-Antibody-(C.15200002)-Chromatin-Immunoprecipitation-Sequencing-NBP2-59208-img0005.jpg)
![Chromatin Immunoprecipitation: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - ChIP results obtained with the antibody against TBP. ChIP assays were performed using U2OS cells, the antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.](http://images.novusbio.com/images2/TATA binding protein TBP Antibody (C.15200002)-Chromatin Immunoprecipitation-NBP2-59208-img0002.jpg)
![Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.](http://images.novusbio.com/images2/EIF3B Antibody-Western Blot-NBP2-24571-img0001.jpg)
![Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/images2/EIF3B-Antibody-Immunohistochemistry-Paraffin-NBP2-24571-img0002.jpg)
![Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/EIF3B-Antibody-Simple-Western-NBP2-24571-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:250. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Paraffin-embedded mouse pancreas. NAGLU antibody diluted at 1:500.](http://images.novusbio.com/images2/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0001.jpg)
![GM-CSF [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GM-CSF [Unconjugated]")
![Immunocytochemistry/Immunofluorescence: SCN7A Antibody [NB100-81029] - Staining of sodium channel protein type 7 subunit alpha in human sperm cells.](http://images.novusbio.com/images2/SCN7A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81029-img0002.jpg)
![Immunohistochemistry-Paraffin: SCN7A Antibody [NB100-81029] - Staining of normal human heart tissue formalin fixed and paraffin embedded.](http://images.novusbio.com/images2/SCN7A-Antibody-Immunohistochemistry-Paraffin-NB100-81029-img0001.jpg)
![Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/HMBS-Antibody-Western-Blot-NBP1-87329-img0006.jpg)
![Immunohistochemistry-Paraffin: HMBS Antibody [NBP1-87329] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/images2/HMBS-Antibody-Immunohistochemistry-Paraffin-NBP1-87329-img0009.jpg)
![Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/HMBS-Antibody-Western-Blot-NBP1-87329-img0005.jpg)
![Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.](http://images.novusbio.com/images2/RAPGEF5 Antibody-Western Blot-NBP2-57362-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [NBP2-57362] - Staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/images2/RAPGEF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57362-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0010.jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Flow-Cytometry-NB120-6405-img0007.jpg)
![TNF-alpha [HRP]](http://images.novusbio.com/images2/TNFalpha_MTA00_ELISA_1036.jpg "TNF-alpha [HRP]")
![beta 2-Microglobulin [Biotin]](http://images.novusbio.com/images2/beta_2Microglobulin_KGE019_ELISA_1152.jpg "beta 2-Microglobulin [Biotin]")