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beta 2-Microglobulin Antibody (747502) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clone
747502
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
FAB3864V-100UG
Alexa Fluor 594
FAB3864T-100UG
Alexa Fluor 647
FAB3864R-100UG
Alexa Fluor 700
FAB3864N-100UG
Alexa Fluor 750
FAB3864S-100UG
Unconjugated
MAB3864
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3864
Carrier Free
MAB3864
Carrier Free
MAB3864-SP
Carrier Free
FAB3864R-100UG
Carrier Free
FAB3864N-100UG
Carrier Free
FAB3864V-100UG
Carrier Free
FAB3864T-100UG
Carrier Free
FAB3864S-100UG
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beta 2-Microglobulin Antibody (747502) [Alexa Fluor® 350] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat beta 2-Microglobulin
Ile21-Met119
Accession # P07151
Specificity
Detects rat beta 2-Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 2-Macroglobulin is observed.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta 2-Microglobulin Antibody (747502) [Alexa Fluor® 350]

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

beta 2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat  beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
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Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
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Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
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TNF-alpha
[Unconjugated]
210-TA
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C-Reactive
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IL-6 [HRP]
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HMBS Antibody
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Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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RAPGEF5 Antibody
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MHC Class I
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB3864U
Alexa Fluor 405 FAB3864V
Alexa Fluor 594 FAB3864T
Alexa Fluor 647 FAB3864R
Alexa Fluor 700 FAB3864N
Alexa Fluor 750 FAB3864S
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol B2M
Uniprot