Beclin 1 Antibody (9B6)

Images

 
Western Blot: Beclin 1 Antibody (9B6) [NBP1-00088] - HeLa whole cell extract.
Western Blot: Beclin 1 Antibody (9B6) [NBP1-00088] - Beclin 1 is acetylated at lysines 430 and 437. Endogenous Beclin 1 is acetylated. Acetylated proteins were immunoprecipitated with the antibody to acetylated lysine ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Eq, PmSpecies Glossary
Applications WB
Clone
9B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Beclin 1 Antibody (9B6) Summary

Immunogen
Full length human Beclin 1 protein [Swiss-Prot# Q14457]
Localization
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BECN1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This Beclin 1 antibody is useful for IHC and WB, where a band is seen ~52 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Beclin 1 Overexpression Lysate
HeLa Whole Cell Lysate
Beclin 1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP1-00088 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Beclin 1 Antibody (9B6)

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • Beclin 1
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Beclin 1 Antibody (NBP1-00088)(3)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
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(1)
Mouse
(1)
Bovine
(1)
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Product General Protocols

View specific protocols for Beclin 1 Antibody (NBP1-00088): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Beclin 1 Antibody (NBP1-00088) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on Beclin 1. Showing 1-10 of 15 blog posts - Show all blog posts.


  Read full blog post.

E-syt in Autophagosome biogenesis: What is the source of it all?
By Christina Towers, PhD. Macroautophagy is a cellular recycling process that requires the formation of double membrane structures to engulf and degrade damaged cytoplasmic material. The pathway involves over 20 co...  Read full blog post.

Lysosomal Dysfunction is Linked to Exosomal Secretion
By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis....  Read full blog post.

CaMKII stimulates autophagic degradation of 'ID', a new frontier against cancer
By Yoskaly Lazo-Fernandez, PhD The field of Cancer Stem Cell (CSC) research has been gaining traction in recent years1. CSCs are a minority group of cells (usually about 1 in 10000) within solid tumors of hematolog...  Read full blog post.


  Read full blog post.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol BECN1
Entrez
Uniprot