Beclin 1 Antibody (4H10)

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Beclin 1 Antibody (4H10) Summary

• Immunogen: Full length human Beclin 1 protein [Swiss-Prot# Q14457]
• Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
• Predicted Species: Mouse (98%), Rat (97%), Porcine (97%), Bovine (98%), Primate (99%), Equine (97%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BECN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50- 1:100
  • Immunohistochemistry-Paraffin 1:50- 1:100
  • Immunoprecipitation
  • Western Blot 2 ug/ml
• Application Notes: This Beclin 1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western Blot, where a band is seen ~52 kDa.. Use in immunprecipitation reported in scientific literature (PMID 25127057) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Beclin 1 Overexpression Lysate

Reactivity Notes

Human. Chicken reactivity reported in scientific literature (PMID: 29706614). 99% sequence identity with monkey, 98% identity with mouse and bovine, 97% identity with porcine, rat and horse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Beclin 1 Antibody (4H10)

• ATG6 autophagy related 6 homolog
• ATG6
• beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
• beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
• Beclin 1
• beclin 1, autophagy related
• beclin1
• beclin-1
• BECN1
• Coiled-coil myosin-like BCL2-interacting protein
• GT197
• Protein GT197
• VPS30

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beclin 1 Antibody (NBP1-00085)(19)

Publications using NBP1-00085

• Zhang Y, Jiang Q, Xie S et al. Lead Induced Ototoxicity and Neurotoxicity in Adult Guinea Pig BioMed Research International 2019-01-15 [PMID: 30766882] (WB, Guinea Pig)
• Yan Q, Song Y, Zhang L et al. Autophagy activation contributes to lipid accumulation in tubular epithelial cells during kidney fibrosis. Cell Death Discov. 2018-06-27 [PMID: 30062051] (WB, Mouse)
• Lin X, Liu X, Ma Y et al. Coherent apoptotic and autophagic activities involved in regression of chicken postovulatory follicles. Aging (Albany NY) 2018-04-29 [PMID: 29706614] (IHC-P, Chicken)
• Wei ZB, Yuan YF, Jaouen F et al. SLC35D3 increases autophagic activity in midbrain dopaminergic neurons by enhancing BECN1-ATG14-PIk3C3 complex formation. Autophagy. 2016-05-12 [PMID: 27171858]
• Morikawa A, Takeuchi T, Kito Y et al. Expression of beclin-1 in the microenvironment of invasive ductal carcinoma of the breast: correlation with prognosis and the cancer-stromal interaction. PLoS ONE. 2015-05-09 [PMID: 25955408] (WB, IHC-P, Human)
• Mandell MA, Jain A, Arko-Mensah J et al. TRIM Proteins Regulate Autophagy and Can Target Autophagic Substrates by Direct Recognition. Dev. Cell. 2014-08-25 [PMID: 25127057] (IP, WB, Human)
• Avena P, Anselmo W, Whitaker-Menezes D et al. Compartment-specific activation of PPARgamma governs breast cancer tumor growth, via metabolic reprogramming and symbiosis. Cell Cycle 2013-05-01 [PMID: 23574724]
• Al-Zoubi M, Salem AF, Martinez-Outschoorn UE et al. Creating a tumor-resistant microenvironment: Cell-mediated delivery of TNFalpha completely prevents breast cancer tumor formation in vivo. Cell Cycle 2013-02-01 [PMID: 23292149] (WB, Human)
• Martinez-Outschoorn UE, Lin Z, Whitaker-Menezes D et al. Ketone body utilization drives tumor growth and metastasis. Cell Cycle 2012-11-01 [PMID: 23082722] (WB, Human)
• Carito V, Bonuccelli G, Martinez-Outschoorn UE et al. Metabolic remodeling of the tumor microenvironment: Migration stimulating factor (MSF) reprograms myofibroblasts toward lactate production, fueling anabolic tumor growth Cell Cycle 2012-09-15 [PMID: 22918248] (WB, Human)
• Capparelli C, Whitaker-Menezes D, Guido C et al. CTGF drives autophagy, glycolysis and senescence in cancer-associated fibroblasts via HIF1 activation, metabolically promoting tumor growth Cell Cycle 2012-06-15 [PMID: 22684333] (WB, Human)
• Salem AF, Whitaker-Menezes D, Howell A et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle 2012-11-15 [PMID: 23070475]
• Salem A, Whitaker-Menezes D, Lin Z et al. Two-compartment tumor metabolism: autophagy in the tumor microenvironment and oxidative mitochondrial metabolism (OXPHOS) in cancer cells. Cell Cycle. 2012-01-01 [PMID: 22722266]
• Capparelli C, Guido C, Whitaker-Menezes D et al. Autophagy and senescence in cancer-associated fibroblasts metabolically supports tumor growth and metastasis via glycolysis and ketone production Cell Cycle 2012-06-01 [PMID: 22684298] (WB, Human)
• Ko YH, Lin Z, Flomenberg N et al. Glutamine fuels a vicious cycle of autophagy in the tumor stroma and oxidative mitochondrial metabolism in epithelial cancer cells: implications for preventing chemotherapy resistance. Cancer Biol Ther. 2011-12-15 [PMID: 22236876] (WB, Human)
• Chiavarina B, Whitaker-Menezes D, Martinez-Outschoorn UE et al. Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth Cancer Biol Ther 2011-12-15 [PMID: 22236875] (WB, Human)
• Martinez-Outschoorn UE, Trimmer C, Lin Z et al. Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of hypoxia, HIF1 induction and NFkappaB activation in the tumor stromal microenvironment. Cell Cycle 2010-09-01 [PMID: 20855962]

Review for Beclin 1 Antibody (NBP1-00085) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/12/2014

Summary

• Application: Western Blot
• Species: Human
• Lot: B-1

Bioinformatics

• Gene Symbol: BECN1