Beclin 1 Antibody (4H10)


Western Blot: Beclin 1 Antibody (4H10) [NBP1-00085] - Beclin 1/ATG6 Antibody (4H10) [NBP1-00085] - Analysis of Beclin 1 in 1. NIH3T3 cell lysate and 2. Cos7 cell lysate.
Immunohistochemistry-Paraffin: Beclin 1/ATG6 Antibody (4H10) [NBP1-00085] - Staining of Beclin 1 in mouse lung.
Western Blot: Beclin 1/ATG6 Antibody (4H10) [NBP1-00085] - Beclin 1 in pancreatic cancer cells. Image from verfied customer review.

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Beclin 1 Antibody (4H10) Summary

Full length human Beclin 1 protein [Swiss-Prot# Q14457]
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Predicted Species
Mouse (98%), Rat (97%), Porcine (97%), Bovine (98%), Chicken (91%), Primate (99%), Equine (97%). Backed by our 100% Guarantee.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:50- 1:100
  • Immunohistochemistry-Paraffin 1:50- 1:100
  • Immunoprecipitation
Application Notes
This Beclin 1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western Blot, where a band is seen ~52 kDa.. Use in immunprecipitation reported in scientific literature (PMID 25127057) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Beclin 1 Lysate (NBL1-07966)
HeLa Lysate (NB820-59462)
Beclin 1 Lysate (NBP2-66129)
Reviewed Applications
Read 1 Review rated 5
NBP1-00085 in the following applications:

Read Publications using
NBP1-00085 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    10 publications

Reactivity Notes

Human. 99% sequence identity with monkey, 98% identity with mouse and bovine, 97% identity with porcine, rat and horse, 91% identity with chicken.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for Beclin 1 Antibody (4H10)

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • Beclin 1
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30


Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Beclin 1 Antibody (NBP1-00085)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 3 applications: IHC-P, IP, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP1-00085 Applications Species
Yan Q, Song Y, Zhang L et al. Autophagy activation contributes to lipid accumulation in tubular epithelial cells during kidney fibrosis. Cell Death Discov. Jun 27 2018 [PMID: 30062051] (WB, Mouse) WB Mouse
Lin X, Liu X, Ma Y et al. Coherent apoptotic and autophagic activities involved in regression of chicken postovulatory follicles Aging (Albany NY) Apr 29 2018 [PMID: 29706614] (IHC-P, Chicken) IHC-P Chicken
Wei ZB, Yuan YF, Jaouen F et al. SLC35D3 increases autophagic activity in midbrain dopaminergic neurons by enhancing BECN1-ATG14-PIk3C3 complex formation. Autophagy. May 12 2016 [PMID: 27171858]
Morikawa A, Takeuchi T, Kito Y et al. Expression of beclin-1 in the microenvironment of invasive ductal carcinoma of the breast: correlation with prognosis and the cancer-stromal interaction. PLoS ONE. 2015 May 09 [PMID: 25955408] (WB, IHC-P, Human) WB, IHC-P Human
Mandell MA, Jain A, Arko-Mensah J et al. TRIM Proteins Regulate Autophagy and Can Target Autophagic Substrates by Direct Recognition. Dev. Cell. 2014 Aug 25 [PMID: 25127057] (WB, IP, Human) WB, IP Human
Avena P, Anselmo W, Whitaker-Menezes D et al. Compartment-specific activation of PPARgamma governs breast cancer tumor growth, via metabolic reprogramming and symbiosis. Cell Cycle 2013 May 1 [PMID: 23574724]
Al-Zoubi M, Salem AF, Martinez-Outschoorn UE et al. Creating a tumor-resistant microenvironment: Cell-mediated delivery of TNFalpha completely prevents breast cancer tumor formation in vivo. Cell Cycle 2013 Feb 1 [PMID: 23292149] (WB, Human) WB Human
Martinez-Outschoorn UE, Lin Z, Whitaker-Menezes D et al. Ketone body utilization drives tumor growth and metastasis Cell Cycle 2012 Nov 1 [PMID: 23082722] (WB, Human) WB Human
Show All 18 Publications.

Review for Beclin 1 Antibody (NBP1-00085) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-00085:
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Images Ratings Applications Species Date Details
Western Blot Beclin 1 NBP1-00085
reviewed by:
WB Human 05/12/2014


ApplicationWestern Blot
Special ApplicationsThese samples are from pancreatic cancer cells.
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Blogs on Beclin 1. Showing 1-10 of 13 blog posts - Show all blog posts.

Lysosomal Dysfunction is Linked to Exosomal Secretion
By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis....  Read full blog post.

CaMKII stimulates autophagic degradation of 'ID', a new frontier against cancer
By Yoskaly Lazo-Fernandez, PhD The field of Cancer Stem Cell (CSC) research has been gaining traction in recent years1. CSCs are a minority group of cells (usually about 1 in 10000) within solid tumors of hematolog...  Read full blog post.

Brain size matters: MTOR regulates autophagy and number of cortical interneurons
By Jamshed Arslan Pharm.D. Interneurons transmit impulses between other neurons, in part, to facilitate the birth of neurons. Cortical interneurons themselves arise from the progenitors in the ventral telencephalon,...  Read full blog post.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

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Recent Reviews


Application: WB
Species: Human


Gene Symbol BECN1