Beclin 1 Antibody (4H10)

Images

 
Western Blot: Beclin 1/ATG6 Antibody (4H10) [NBP1-00085] - Beclin 1 in pancreatic cancer cells. Image from verfied customer review.
Immunohistochemistry-Paraffin: Beclin 1/ATG6 Antibody (4H10) [NBP1-00085] - Staining of Beclin 1 in mouse lung.
Western Blot: Beclin 1/ATG6 Antibody (4H10) [NBP1-00085] - Analysis of Beclin 1 in 1. NIH3T3 cell lysate and 2. Cos7 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Eq, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
4H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Beclin 1 Antibody (4H10) Summary

Immunogen
Full length human Beclin 1 protein [Swiss-Prot# Q14457]
Localization
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Predicted Species
Mouse (98%), Rat (97%), Porcine (97%), Equine (97%), Bovine (98%), Primate (99%), Chicken (91%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BECN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:50- 1:100
  • Immunohistochemistry-Paraffin 1:50- 1:100
  • Immunoprecipitation
Application Notes
This Beclin 1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western Blot, where a band is seen ~52 kDa.. Use in immunprecipitation reported in scientific literature (PMID 25127057)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Beclin 1 Lysate (NBL1-07966)
HeLa Lysate (NB820-59462)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-00085 in the following applications:

Publications
Read Publications using
NBP1-00085 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    9 publications

Reactivity Notes

Human. 99% sequence identity with monkey, 98% identity with mouse and bovine, 97% identity with porcine, rat and horse, 91% identity with chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Beclin 1 Antibody (4H10)

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • Beclin 1
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Beclin 1 Antibody (NBP1-00085)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC-P, IP, WB.


Filter By Application
IHC-P
(1)
IP
(1)
WB
(9)
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Filter By Species
Human
(9)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-00085 Applications Species
Wei ZB, Yuan YF, Jaouen F et al. SLC35D3 increases autophagic activity in midbrain dopaminergic neurons by enhancing BECN1-ATG14-PIk3C3 complex formation. Autophagy. May 12 2016 [PMID: 27171858]
Morikawa A, Takeuchi T, Kito Y et al. Expression of beclin-1 in the microenvironment of invasive ductal carcinoma of the breast: correlation with prognosis and the cancer-stromal interaction. PLoS ONE. 2015 May 09 [PMID: 25955408] (WB, IHC-P, Human) WB, IHC-P Human
Mandell MA, Jain A, Arko-Mensah J et al. TRIM Proteins Regulate Autophagy and Can Target Autophagic Substrates by Direct Recognition. Dev. Cell. 2014 Aug 25 [PMID: 25127057] (WB, IP, Human) WB, IP Human
Avena P, Anselmo W, Whitaker-Menezes D et al. Compartment-specific activation of PPARgamma governs breast cancer tumor growth, via metabolic reprogramming and symbiosis. Cell Cycle 2013 May 1 [PMID: 23574724]
Al-Zoubi M, Salem AF, Martinez-Outschoorn UE et al. Creating a tumor-resistant microenvironment: Cell-mediated delivery of TNFalpha completely prevents breast cancer tumor formation in vivo. Cell Cycle 2013 Feb 1 [PMID: 23292149] (WB, Human) WB Human
Martinez-Outschoorn UE, Lin Z, Whitaker-Menezes D et al. Ketone body utilization drives tumor growth and metastasis Cell Cycle 2012 Nov 1 [PMID: 23082722] (WB, Human) WB Human
Salem AF, Whitaker-Menezes D, Howell A et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle 2012 Nov 15 [PMID: 23070475]
Carito V, Bonuccelli G, Martinez-Outschoorn UE et al. Metabolic remodeling of the tumor microenvironment: Migration stimulating factor (MSF) reprograms myofibroblasts toward lactate production, fueling anabolic tumor growth Cell Cycle 2012 Sep 15 [PMID: 22918248] (WB, Human) WB Human
Salem A, Whitaker-Menezes D, Lin Z et al. Two-compartment tumor metabolism: autophagy in the tumor microenvironment and oxidative mitochondrial metabolism (OXPHOS) in cancer cells. Cell Cycle. 2012 [PMID: 22722266]
Capparelli C, Whitaker-Menezes D, Guido C et al. CTGF drives autophagy, glycolysis and senescence in cancer-associated fibroblasts via HIF1 activation, metabolically promoting tumor growth Cell Cycle 2012 Jun 15 [PMID: 22684333] (WB, Human) WB Human
Show All 16 Publications.

Review for Beclin 1 Antibody (NBP1-00085) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-00085:
Filter by Applications
WB
(1)
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Filter by Species
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(1)
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Images Ratings Applications Species Date Details
Western Blot Beclin 1 NBP1-00085
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reviewed by:
Anonymous
WB Human 05/12/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
LotB-1
Special ApplicationsThese samples are from pancreatic cancer cells.
FileView PDF

Product General Protocols

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FAQs for Beclin 1 Antibody (NBP1-00085) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-00085AF405
Alexa Fluor 488 Labeled NBP1-00085AF488
Alexa Fluor 647 Labeled NBP1-00085AF647
Alexa Fluor 700 Labeled NBP1-00085AF700
Biotin Labeled NBP1-00085B
DyLight 405 Labeled NBP1-00085V
DyLight 488 Labeled NBP1-00085G
DyLight 550 Labeled NBP1-00085R
DyLight 650 Labeled NBP1-00085C
FITC Labeled NBP1-00085F
HRP Labeled NBP1-00085H

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Bioinformatics Tool for Beclin 1 Antibody (NBP1-00085)

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Blogs on Beclin 1.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Wide Ranging Uses for the Autophagy Marker - Beclin-1 Antibody
Beclin 1 is the first mammalian gene identified as a mediator of autophagy, and plays important roles in development, tumorigenesis, and neurodegeneration. Research with the beclin-1 antibody has revealed that, Beclin 1 is found in complex with the th...  Read full blog post.

Beclin 1: Regulator of Autophagy and Apoptosis
Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul...  Read full blog post.

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Recent Reviews

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Anonymous
05/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol BECN1
Entrez