BCL9-2 Antibody - BSA Free

Western Blot: BCL9-2 Antibody [NBP2-14350] - Analysis in human cell lines A-549 and HEK293 using anti-BCL9L antibody. Corresponding BCL9L RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.

Immunocytochemistry/Immunofluorescence: BCL9-2 Antibody [NBP2-14350] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350] - Staining of human breast shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BCL9-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCL9-2 Antibody - BSA Free (NBP2-14350) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: MSMCHPGQMSLLGRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVGGEVYSQPPHMLSPQGSLMGPPPQQNLMVSHPLRQRSVSLDSQMGYLPAP
• Predicted Species: Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL9L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  BCL9-2 Protein (NBP2-14350PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BCL9-2 Antibody - BSA Free

• B-cell CLL/lymphoma 9-like protein
• B-cell CLL/lymphoma 9-like
• B-cell lymphoma 9-like protein
• Bcl92
• Bcl9-2
• BCL9-like protein
• DLNB11
• DLNB11BCL9-2
• nuclear co-factor of beta-catenin signalling
• Protein BCL9-2

Background

BCL9L - B-cell CLL/lymphoma 9-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL9L