Bcl-6 Antibody (CL14611) - Azide and BSA Free

Staining in A-549 cell line with Anti-Bcl-6 monoclonal antibody) .

Analysis in human lymph node and small intestine tissues using NBP3-44142 antibody. Corresponding Bcl-6 RNA-seq data are presented for the same tissues.

Staining of human cervix shows moderate nuclear positivity in squamous epithelial cells.

Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.

Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.

Staining of human small intestine shows weak nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL14611
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Bcl-6 Antibody (CL14611) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Bcl-6 Antibody (CL14611) - Azide and BSA Free (NBP3-26872) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P41182, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Bcl-6 Antibody (CL14611) - Azide and BSA Free

• B-cell CLL/lymphoma 6
• B-cell lymphoma 6 protein transcript
• Bcl-5
• BCL5B-cell lymphoma 5 protein
• Bcl6
• Bcl-6
• BCL6A
• cys-his2 zinc finger transcription factor
• LAZ3
• LAZ3ZBTB27B-cell lymphoma 6 protein
• lymphoma-associated zinc finger gene on chromosome 3
• Protein LAZ-3
• ZBTB27
• Zinc finger and BTB domain-containing protein 27
• Zinc finger protein 51ZNF51
• zinc finger transcription factor BCL6S
• ZNF51

Background

Bcl6 reacts with Bcl6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL6