Bcl-10 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Bcl-10 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human BCL10.
  
  

  Source: E. coli

  Amino Acid Sequence: MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRT

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: BCL10
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85697.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Bcl-10 Recombinant Protein Antigen

• B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10
• Bcl10
• Bcl-10
• CARD-containing apoptotic signaling protein
• CARD-containing molecule enhancing NF-kappa-B
• CARD-containing proapoptotic protein
• CARD-like apoptotic protein
• CARMEN
• caspase-recruiting domain-containing protein
• cCARMEN
• c-E10
• c-E10bcl-10
• CED-3/ICH-1 prodomain homologous E10-like regulator
• Cellular homolog of vCARMEN
• cellular-E10
• CIPER
• CLAP
• hCLAP
• Mammalian CARD-containing adapter molecule E10
• mE10
• mE10CARD containing molecule enhancing NF-kB

Background

Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB (1-2). Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BCL10