BCKDK Antibody (OTI11C9)

Western Blot: BCKDK Antibody (11C9) [NBP1-47664] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.

Immunocytochemistry/Immunofluorescence: BCKDK Antibody (11C9) [NBP1-47664] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.

Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human Ovary tissue using anti-BCKDK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human endometrium tissue using anti-BCKDK mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI11C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.8 mg/ml

BCKDK Antibody (OTI11C9) Summary

• Description: Novus Biologicals Mouse BCKDK Antibody (OTI11C9) (NBP1-47664) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-BCKDK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCKDK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 46.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32561715). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.8 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCKDK Antibody (OTI11C9)

• BCKDHKIN
• branched chain alpha-ketoacid dehydrogenase kinase
• branched chain ketoacid dehydrogenase kinase
• EC 2.7.11
• EC 2.7.11.4
• mitochondrial

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCKDK Antibody (NBP1-47664)(1)

Publication using NBP1-47664

• Son SM, Park SJ, Stamatakou E et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor Nat Commun 2020-06-19 [PMID: 32561715] (WB, Mouse)

Bioinformatics

• Gene Symbol: BCKDK
• Entrez:
  • 10295(Human)