BCCIP Antibody - BSA Free

Western Blot: BCCIP Antibody [NBP1-31319] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: BCCIP Antibody [NBP1-31319] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: BCCIP Antibody [NBP1-31319] - BCCIP antibody detects BCCIP protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. BCCIP antibody dilution: 1:500. Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: BCCIP Antibody [NBP1-31319] - Sample: Paraffin-embedded BT483 xenograft. BCCIP antibody dilution: 1:500.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BCCIP Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCCIP. The exact sequence is proprietary.
• Localization: Nucleus |
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCCIP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BCCIP Antibody - BSA Free

• BCCIPalpha
• BRCA2 and CDKN1A interacting protein
• BRCA2 and CDKN1A-interacting protein
• BRCA2 and Cip1/p21 interacting protein
• cdk inhibitor p21 binding protein
• P21- and CDK-associated protein 1
• Protein TOK-1
• TOK-1
• TOK-1alpha
• TOK1BCCIPbeta
• TOK-1beta

Background

This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BCCIP