BCAT1 Antibody (OTI3F5)

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon)

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.

BCAT1 Antibody (OTI3F5)[NBP2-01826]Simple Western� analysis of endogenous protein IFIT3 from THP-1 lysates (0.1 mg/mL) using IFIT3 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

• Reactivity: Hu, Mu, Ca
• Applications: WB, IHC
• Clone: OTI3F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BCAT1 Antibody (OTI3F5) Summary

• Description: Novus Biologicals Mouse BCAT1 Antibody (OTI3F5) (NBP2-01826) is a monoclonal antibody validated for use in IHC and WB. Anti-BCAT1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 42.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BCAT1 Antibody (OTI3F5)

• BCAT(c)
• BCAT1
• BCAT-1
• BCATC
• BCT1
• BCT1PNAS121
• branched chain amino-acid transaminase 1, cytosolic
• branched chain aminotransferase 1, cytosolic
• branched-chain-amino-acid aminotransferase, cytosolic
• DKFZp686E12175
• EC 2.6.1.42
• ECA39
• MECA39
• placental protein 18
• PNAS121
• PP18
• Protein ECA39

Background

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAT1 Antibody (NBP2-01826)(2)

Publications using NBP2-01826

• Zhu Z, Achreja A, Meurs N et al. Tumour-reprogrammed stromal BCAT1 fuels branched-chain ketoacid dependency in stromal-rich PDAC tumours Nat Metab 2020-07-06 [PMID: 32694827] (WB, Human)
• Li H, Ye D, Xie W et al. Defect of Branched-chain Amino Acid Metabolism Promotes the Development of Alzheimer's Disease by Targeting the mTOR Signaling Biosci. Rep. 2018-05-25 [PMID: 29802157] (Mouse)

Bioinformatics

• Gene Symbol: BCAT1
• Entrez:
  • 586(Human)