BCAT1 Antibody (OTI3F5)

Images

 
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI3F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.78 mg/ml

BCAT1 Antibody (OTI3F5) Summary

Immunogen
Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BCAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
42.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-01826 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAT1 Antibody (OTI3F5)

  • BCAT(c)
  • BCAT1
  • BCAT-1
  • BCATC
  • BCT1
  • BCT1PNAS121
  • branched chain amino-acid transaminase 1, cytosolic
  • branched chain aminotransferase 1, cytosolic
  • branched-chain-amino-acid aminotransferase, cytosolic
  • DKFZp686E12175
  • EC 2.6.1.42
  • ECA39
  • MECA39
  • placental protein 18
  • PNAS121
  • PP18
  • Protein ECA39

Background

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-33705
Species: Hu
Applications: WB, IHC, IHC-P
NB600-302
Species: Hu, Mu, Bv, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Flow-IC
NBP1-26618
Species: Hu
Applications: WB, ChIP, IHC, IHC-P, IP
NBP2-32887
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-52455
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-93638
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-16134
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-33471
Species: Hu
Applications: IHC, IHC-P
NBP1-87724
Species: Hu
Applications: WB, IHC, IHC-P
NB600-607
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
H00003845-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IP
NBP1-82493
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KO
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB300-558
Species: Hu, Bv, Rb
Applications: WB, ELISA, ICC/IF, IP
NBP2-01308
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for BCAT1 Antibody (NBP2-01826)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for BCAT1 Antibody (NBP2-01826) (0)

There are no reviews for BCAT1 Antibody (NBP2-01826). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BCAT1 Antibody (NBP2-01826) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70242AF350
Alexa Fluor 405 NBP2-70242AF405
Alexa Fluor 488 NBP2-70242AF488
Alexa Fluor 532 NBP2-70242AF532
Alexa Fluor 594 NBP2-70242AF594
Alexa Fluor 647 NBP2-70242AF647
Alexa Fluor 700 NBP2-70242AF700
Alexa Fluor 750 NBP2-70242AF750
Biotin NBP2-70242B
DyLight 350 NBP2-70242UV
DyLight 405 NBP2-70242V
DyLight 488 NBP2-70242G
DyLight 550 NBP2-70242R
DyLight 594 NBP2-70242DL594
DyLight 650 NBP2-70242C
DyLight 680 NBP2-70242FR
DyLight 755 NBP2-70242IR
FITC NBP2-70242F
HRP NBP2-70242H
Janelia Fluor 549 NBP2-70242JF549
Janelia Fluor 646 NBP2-70242JF646

Additional BCAT1 Products

Bioinformatics Tool for BCAT1 Antibody (NBP2-01826)

Discover related pathways, diseases and genes to BCAT1 Antibody (NBP2-01826). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BCAT1 Antibody (NBP2-01826)

Discover more about diseases related to BCAT1 Antibody (NBP2-01826).
 

Pathways for BCAT1 Antibody (NBP2-01826)

View related products by pathway.

PTMs for BCAT1 Antibody (NBP2-01826)

Learn more about PTMs related to BCAT1 Antibody (NBP2-01826).
 

Research Areas for BCAT1 Antibody (NBP2-01826)

Find related products by research area.

Blogs on BCAT1

There are no specific blogs for BCAT1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BCAT1 Antibody (OTI3F5) and receive a gift card or discount.

Bioinformatics

Gene Symbol BCAT1
Entrez