BCAT1 Antibody (1F8) - Azide and BSA Free

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02] - BCAT1 monoclonal antibody (M02), clone 1F8. Analysis of BCAT1 expression in human kidney.

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02] - BCAT1 monoclonal antibody (M02), clone 1F8. Analysis of BCAT1 expression in MCF-7.

Sandwich ELISA: BCAT1 Antibody (1F8) [H00000586-M02] - Detection limit for recombinant GST tagged BCAT1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BCAT1 Antibody (1F8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: BCAT1 (AAH33864, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF
• Specificity: BCAT1 - branched chain aminotransferase 1, cytosolic (1F8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCAT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BCAT1 Antibody (1F8) - Azide and BSA Free

• BCAT(c)
• BCAT1
• BCAT-1
• BCATC
• BCT1
• BCT1PNAS121
• branched chain amino-acid transaminase 1, cytosolic
• branched chain aminotransferase 1, cytosolic
• branched-chain-amino-acid aminotransferase, cytosolic
• DKFZp686E12175
• EC 2.6.1.42
• ECA39
• MECA39
• placental protein 18
• PNAS121
• PP18
• Protein ECA39

Background

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAT1 Antibody (H00000586-M02)(1)

Publication using H00000586-M02

• Ji D, Jiang C, Zhang L et al. LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis. J Cell Physiol 2018-09-19 [PMID: 30230527]

Bioinformatics

• Gene Symbol: BCAT1
• Entrez:
  • 586(Human)