BCAS2 Antibody

Images

 
Western Blot: BCAS2 Antibody [NBP1-77124] - MCF7 cell lysate with BCAS2 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: BCAS2 Antibody [NBP1-77124] - Immunofluorescence of BCAS2 in human breast carcinoma tissue with BCAS2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NBP1-77124] - Human breast carcinoma with BCAS2 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BCAS2 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BCAS2. The immunogen is located within the last 50 amino acids of BCAS2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Control Peptide
BCAS2 Peptide (NBP1-77124PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAS2 Antibody

  • breast carcinoma amplified sequence 2
  • Breast carcinoma-amplified sequence 2
  • DAM1DNA amplified in mammary carcinoma 1 protein
  • pre-mRNA-splicing factor SPF27
  • Snt309
  • SPF27
  • spliceosome associated protein, amplified in breast cancer
  • Spliceosome-associated protein SPF 27

Background

BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCAS2
Uniprot