BCAS2 Antibody - BSA Free

Western Blot: BCAS2 Antibody [NBP1-77124] - MCF7 cell lysate with BCAS2 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: BCAS2 Antibody - BSA Free [NBP1-77124] - Immunofluorescence of BCAS2 in human breast carcinoma tissue with BCAS2 antibody at 20 u/mL.

Immunohistochemistry: BCAS2 Antibody - BSA Free [NBP1-77124] - Immunohistochemistry of BCAS2 in human breast carcinoma with BCAS2 antibody at 5 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BCAS2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCAS2 Antibody - BSA Free (NBP1-77124) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BCAS2. The immunogen is located within the last 50 amino acids of BCAS2. Amino Acid Squence: QHGEANKENIRQDF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCAS2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  BCAS2 Peptide (NBP1-77124PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BCAS2 Antibody - BSA Free

• breast carcinoma amplified sequence 2
• Breast carcinoma-amplified sequence 2
• DAM1DNA amplified in mammary carcinoma 1 protein
• pre-mRNA-splicing factor SPF27
• Snt309
• SPF27
• spliceosome associated protein, amplified in breast cancer
• Spliceosome-associated protein SPF 27

Background

BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCAS2
• Uniprot:
  • CAG46834(Human)