BCAR3 Antibody

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Western Blot: BCAR3 Antibody [NB300-825] - Analysis of BCAR3 in Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BCAR3 Antibody Summary

Immunogen
Peptide with sequence C-RKLEPPPVKQAEL corresponding to C-Terminus according to NP_003558.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BCAR3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: An anonymous customer has reported an approx. 110 kDa band observed in mouse embryonic kidney epithelial lysates (calculated MW of 92.6 kDa band according to human NP_003558.1 and 92.3 kDa band according to mouse NP_038895.1).
Positive Control
BCAR3 Lysate (NBL1-07936)
Publications
Read Publication using NB300-825.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAR3 Antibody

  • breast cancer estrogen resistance 3
  • dJ1033H22.2 (breast cancer estrogen resistance 3)
  • KIAA0554
  • Novel SH2-containing protein 2
  • NSP2SH2D3Bbreast cancer estrogen resistance protein 3
  • SH2 domain-containing protein 3B

Background

In breast cancer, the antiestrogen tamoxifen has been prescribed for both primary treatment and the treatment of advanced metastatic disease. Although the drug induces remission in most patients with estrogen receptor-positive disease, all patients eventually develop resistance. van Agthoven et al. (1998) applied an insertional mutagenesis strategy to a breast cancer cell line. Infected cells were subjected to tamoxifen selection, and the resistant clones were screened for a common integration site linked with antiestrogen resistance. By screening a human testis cDNA library with the integration site-specific probe, they obtained a cDNA encoding BCAR3 (breast cancer antiestrogen resistance 3). The deduced 825 amino acid BCAR3 protein contains a putative Src homology 2 (SH2) domain and sequences homologous to yeast CDC48. Northern blot analysis detected abundant expression of a 3.4kb BCAR3 transcript in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and several cancer cell lines, but not in nonmalignant breast tissue; a 6kb BCAR3 transcript was also detected in skeletal muscle and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BCAR3 Antibody (NB300-825)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCAR3
Entrez
Uniprot