BCAM/CD239 Antibody [Unconjugated]

Western blot shows lysates of LNCaP human prostate cancer cell line, human prostate tissue, human kidney tissue, and human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BCAM at approximately 70-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Exosome Standards (PC‑3) (NBP2-49856) and human prostate tissue, loaded at 0.5 mg/ml. A specific band was detected for BCAM at approximately 107 kDa (as indicated) using 10 µg/ml of Goat Anti-Human BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF148) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BCAM/CD239 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BCAM
  Glu32-Ala547
  Accession # CAA58449
• Specificity: Detects human BCAM in direct ELISAs and Western blots. In these formats, approximately 2% cross-reactivity with recombinant human (rh) ALCAM is observed and less than 1% cross-reactivity with rhPECAM, rhEpCAM, rhICAM‑1, rhICAM‑2, rhICAM‑3, and rhVCAM‑1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BCAM
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCAM/CD239 Antibody [Unconjugated]

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) residues precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
2. Parsons, S.F. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5496.
3. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
4. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAM/CD239 Antibody (AF148)(6)

Publications using AF148

• Suresh Sivakumar, Sonja Lieber, Damiano Librizzi, Corinna Keber, Leah Sommerfeld, Florian Finkernagel et al. Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer Clinical and Translational Medicine 2023-01-16 [PMID: 36647260]
• TRL Klei, DZ de Back, PJ Asif, PJJH Verkuijlen, M Veldthuis, PC Ligthart, J Berghuis, E Clifford, BM Beuger, TK van den Be, R van Zwiete, W El Nemer, R van Brugge Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging Blood Adv, 2018-01-03;2(1):14-24. 2018-01-03 [PMID: 29344581] (Flow Cytometry, Western Blot, Human)
• M Kim, Y Alapan, A Adhikari, JA Little, UA Gurkan Hypoxia enhanced adhesion of red blood cells in microscale flow Microcirculation, 2017-07-01;0(0):. 2017-07-01 [PMID: 28387057] (Bioassay, Human)
• Rodin S, Antonsson L, Niaudet C, Simonson O, Salmela E, Hansson E, Domogatskaya A, Xiao Z, Damdimopoulou P, Sheikhi M, Inzunza J, Nilsson A, Baker D, Kuiper R, Sun Y, Blennow E, Nordenskjold M, Grinnemo K, Kere J, Betsholtz C, Hovatta O, Tryggvason K Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun, 2014-01-01;5(0):3195. 2014-01-01 [PMID: 24463987] (Neutralization, Human)
• Elin Hadler-Olsen, Premasany Kanapathippillai, Eli Berg, Gunbjørg Svineng, Jan-Olof Winberg, Lars Uhlin-Hansen Gelatin In Situ Zymography on Fixed, Paraffin-embedded Tissue: Zinc and Ethanol Fixation Preserve Enzyme Activity Journal of Histochemistry & Cytochemistry 2010-01-01 [PMID: 19755718]
• Niiya D, Egawa N, Sakamoto T, Kikkawa Y, Shinkawa T, Isobe T, Koshikawa N, Seiki M Identification and characterization of Lutheran blood group glycoprotein as a new substrate of membrane-type 1 matrix metalloproteinase 1 (MT1-MMP): a systemic whole cell analysis of MT1-MMP-associating proteins in A431 cells. J. Biol. Chem., 2009-08-10;284(40):27360-9. 2009-08-10 [PMID: 19667067] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BCAM
• Uniprot:
  • CAA58449()