BCAM/CD239 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

BCAM/CD239 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BCAM
  Glu32-Ala547
  Accession # CAA58449
• Specificity: Detects human BCAM in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ALCAM, rhICAM-1, rhICAM-2, rhICAM-3 and rhCD31 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human BCAM Antibody (Catalog # MAB1481)
  ELISA Detection: Human BCAM Biotinylated Antibody (Catalog # BAF148)
  Standard: Recombinant Human BCAM Fc Chimera (Catalog # 148-BC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCAM/CD239 Antibody [Biotin]

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) residues precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
2. Parsons, S.F. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5496.
3. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
4. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAM/CD239 Antibody (BAF148)(3)

Publications using BAF148

• Franco , Melanie, Collec , Emmanuel, Connes , Philippe, van den Akker , Emile, Billette de Villemeur , Thierry, Belmatoug , Nadia, von Lindern , Marieke, Ameziane , Nejma, Hermine , Olivier, Colin , Yves, Le Van Kim , Caroline, Mignot , Cyril Abnormal properties of red blood cells suggest a role in the pathophysiology of Gaucher disease. Blood, 2012-12-03;121(3):546-55. 2012-12-03 [PMID: 23212518] (Western Blot, Human)
• Collec E, Lecomte MC, El Nemer W, Colin Y, Le Van Kim C Novel role for the Lu/BCAM-spectrin interaction in actin cytoskeleton reorganization. Biochem. J., 2011-06-15;436(3):699-708. 2011-06-15 [PMID: 21434869] (Western Blot, Human)
• Bartolucci P, Chaar V, Picot J, Bachir D, Habibi A, Fauroux C, Galacteros F, Colin Y, Le Van Kim C, El Nemer W Decreased sickle red blood cell adhesion to laminin by hydroxyurea is associated with inhibition of Lu/BCAM protein phosphorylation. Blood, 2010-06-21;116(12):2152-9. 2010-06-21 [PMID: 20566895] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BCAM
• Uniprot:
  • CAA58449()