BAT3/BAG6 Overexpression Lysate (Native)

Images

 
Western Blot: BAT3 Overexpression Lysate (Adult Normal) [NBL1-07919] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BAT3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BAT3/BAG6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201122 and based on accession number NM_080702. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens HLA-B associated transcript 3 (BAT3), transcript variant 2, mRNA.
Gene
BAG6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
118.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BAT3/BAG6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BAT3/BAG6 Overexpression Lysate (Native)

  • BAG6
  • BAT3
  • BAT3HLA-B associated transcript-3
  • BCL2-associated athanogene 6BAG-6
  • D6S52E
  • D6S52EHLA-B-associated transcript 3
  • G3BAG family molecular chaperone regulator 6
  • HLA-B associated transcript 3
  • large proline-rich protein BAT3
  • Protein G3
  • Protein Scythe
  • Scythe

Background

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and utilizes an alternative in-frame splice site in the 5' coding region, compared to variant 1. Variants 2, 3, and 4 encode the same isoform (b), which is 6 aa shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-56762
Species: Hu
Applications: WB, ICC/IF
H00001192-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF5415
Species: Hu
Applications: WB, IHC, ICC
NBP1-89285
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-07799
Species: Hu
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP1-85432
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-36776
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for BAT3/BAG6 Lysate (NBL1-07919) (0)

There are no publications for BAT3/BAG6 Lysate (NBL1-07919).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BAT3/BAG6 Lysate (NBL1-07919) (0)

There are no reviews for BAT3/BAG6 Lysate (NBL1-07919). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BAT3/BAG6 Lysate (NBL1-07919) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional BAT3/BAG6 Products

Bioinformatics Tool for BAT3/BAG6 Lysate (NBL1-07919)

Discover related pathways, diseases and genes to BAT3/BAG6 Lysate (NBL1-07919). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BAT3/BAG6 Lysate (NBL1-07919)

Discover more about diseases related to BAT3/BAG6 Lysate (NBL1-07919).
 

Pathways for BAT3/BAG6 Lysate (NBL1-07919)

View related products by pathway.

PTMs for BAT3/BAG6 Lysate (NBL1-07919)

Learn more about PTMs related to BAT3/BAG6 Lysate (NBL1-07919).

Blogs on BAT3/BAG6

There are no specific blogs for BAT3/BAG6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

BAT3/BAG6 Antibody
30230002-0.1mg

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BAT3/BAG6 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol BAG6
Entrez