BAT3/BAG6 Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

BAT3/BAG6 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human BAT3/BAG6
  Met1-Gln190
  Accession # P46379
• Specificity: Detects human, mouse, and rat BAT3/BAG6 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAT3/BAG6 Antibody [Alexa Fluor® 532]

• BAG6
• BAT3
• BAT3HLA-B associated transcript-3
• BCL2-associated athanogene 6BAG-6
• D6S52E
• D6S52EHLA-B-associated transcript 3
• G3BAG family molecular chaperone regulator 6
• HLA-B associated transcript 3
• large proline-rich protein BAT3
• Protein G3
• Protein Scythe
• Scythe

Background

BAT3 (HLA-B-associated transcript 3; also BAG6, BAG family molecular chaperone regulator 6, Protein G3 and Scythe) is a multifunctional molecule that is not easily placed into known protein families. Although its predicted MW is 119 kDa, it runs anomalously in SDS-PAGE at 150-170 kDa. BAT3 is found in diverse cell types such as dendritic cells, hepatocytes, renal epithelial cells and tumor cells. It is found extracellularly where it is complexed to Hsp70, and activates the NKp30 receptor expressed on NK cells. Intracellularly, it binds to TGF-beta receptors I and II where, upon receptor ligation, it translocates to the nucleus and activates TGF-beta target genes. Human BAT3 is 1132 amino acids (aa) in length. It contains one Ub-like domain (aa 17-77), two Pro-rich regions (aa 195-273 and 394-681), an NLS (aa 1030-1052) plus a BAG domain (aa 1053-1101). BAT3 is phosphorylated on multiple Ser/Thr residues. Caspase 3 will cleave BAT3 after Asp1001, generating a 20 kDa C-terminal fragment. There are also multiple splice variants. Two of them share an absence of aa 185-190 that may be accompanied by a 36 aa insertion after Gly489. Over aa 1-190, human BAT3 shares 93% aa identity with mouse BAT3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.