Bassoon Antibody (SAP7F407)

Western Blot: Bassoon Antibody (SAP7F407) [NBP2-89079] - Western Blot analysis of NBP2-89079: Lane 1: NovexTM HiMarkTM MW Marker, Lane 2: Mouse Brain lysate, Lane 3: Rat Brain lysate

Immunohistochemistry-Paraffin: Bassoon Antibody (SAP7F407) [NBP2-89079] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded rat brain tissue stained with Bassoon monoclonal antibody (SAP7F407) at 10ug/ml.

Product Discontinued

Bassoon Antibody (SAP7F407) Summary

• Immunogen: Recombinant rat Bassoon.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Western Blot: 1:1000, ECL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS , pH 7.2, containing 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Bassoon Antibody (SAP7F407)

• bassoon (presynaptic cytomatrix protein)

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Bassoon Antibody (NBP2-89079). (Showing 1 - 1 of 1 FAQ).

1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
   • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

Bioinformatics

• Gene Symbol: BSN