Band 3 Antibody (BIII 136)

Western Blot: Band 3 Antibody (BIII 136) [NB120-11012] - Human erythrocytes ghosts extract was separated on SDS-PAGE and probed with Monoclonal Anti-Band 3 Clone: BIII-136. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and AEC substrate.

Product Discontinued

Band 3 Antibody (BIII 136) Summary

• Immunogen: Glycophorin B from human erythrocytes.
• Epitope: The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kD from the N-terminal end.
• Localization: Cell Membrane; multi-pass membrane protein
• Specificity: The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kDa from the N-terminal end. This recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC4A1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000
• Application Notes: Immunofluorescent staining of fixed human erythrocytes. WB: Use at an assay dependent dilution. The antibody titer (1:5,000) was determined by indirect immunoblotting using an extract of human erythrocyte ghosts. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins.Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Band 3 Antibody (BIII 136)

• AE 1
• AE1MGC126619
• Anion exchange protein 1
• Anion exchanger 1
• band 3 anion transport protein
• BND3
• CD233 antigen
• CD233
• DI
• EMPB3
• EPB3MGC126623
• erythrocyte membrane protein band 3
• erythroid anion exchange protein
• FR
• Froese blood group
• MGC116750
• MGC116753
• RTA1A
• Solute carrier family 4 member 1
• solute carrier family 4, anion exchanger, member 1 (erythrocyte membraneprotein band 3, Diego blood group)
• solute carrier family 4, anion exchanger, number 1
• SW
• Swann blood group
• Waldner blood group
• WD
• WD1
• WR
• Wright blood group

Background

The protein encoded by the Band 3 gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC4A1
• Entrez:
  • 6521(Human)
  • 20533(Mouse)
• Uniprot:
  • P02730(Human)