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Band 3 Antibody (BIII 136)

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Western Blot: Band 3 Antibody (BIII 136) [NB120-11012] - Human erythrocytes ghosts extract was separated on SDS-PAGE and probed with Monoclonal Anti-Band 3 Clone: BIII-136. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and AEC substrate.
Western Blot: Band 3 Antibody (BIII 136) [NB120-11012] - Human erythrocytes ghosts extract was separated on SDS-PAGE and probed with Monoclonal Anti-Band 3 Clone: BIII-136. The antibody was developed using Goat ...read more

Product Details

Summary
Reactivity Hu, Bv(-), Ca(-), Eq(-), Gp(-), Mu(-), Po(-)Species Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
BIII 136
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Band 3 Antibody (BIII 136) Summary

Immunogen
Glycophorin B from human erythrocytes.
Epitope
The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kD from the N-terminal end.
Localization
Cell Membrane; multi-pass membrane protein
Specificity
The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kDa from the N-terminal end. This recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC4A1
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunofluorescent staining of fixed human erythrocytes. WB: Use at an assay dependent dilution. The antibody titer (1:5,000) was determined by indirect immunoblotting using an extract of human erythrocyte ghosts. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins.Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Band 3 Antibody (BIII 136)

  • AE 1
  • AE1MGC126619
  • Anion exchange protein 1
  • Anion exchanger 1
  • band 3 anion transport protein
  • BND3
  • CD233 antigen
  • CD233
  • DI
  • EMPB3
  • EPB3MGC126623
  • erythrocyte membrane protein band 3
  • erythroid anion exchange protein
  • FR
  • Froese blood group
  • MGC116750
  • MGC116753
  • RTA1A
  • Solute carrier family 4 member 1
  • solute carrier family 4, anion exchanger, member 1 (erythrocyte membraneprotein band 3, Diego blood group)
  • solute carrier family 4, anion exchanger, number 1
  • SW
  • Swann blood group
  • Waldner blood group
  • WD
  • WD1
  • WR
  • Wright blood group

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SLC4A1
Entrez
Uniprot