Bag-1 Antibody

Product Discontinued

Bag-1 Antibody Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human BAG-1. The immunogen is located within the first 50 amino acids of BAG-1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 - 2 mg/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Bag-1 Antibody

• BAG family molecular chaperone regulator 1
• Bag1
• Bag-1
• Bcl-2 associating athanogene-1 protein
• Bcl-2-associated athanogene 1
• BCL2-associated athanogene
• Bcl-2-binding protein
• glucocortoid receptor-associated protein RAP46,46-KD
• HAP
• RAP46

Background

Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAG1
• Uniprot:
  • NP_004314(Human)