Recombinant Human BAFF/BLyS/TNFSF13B Protein

SDS-Page: Recombinant Human BAFF/BLyS/TNFSF13B Protein [NBP2-34883]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human BAFF/BLyS/TNFSF13B Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to BAFF/BLyS/TNFSF13B Source: E. coli

  Uniprot ID: Q9Y275

  Amino Acid Sequence: MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF13B
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of BAFF/BLyS/TNFSF13B as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human BAFF/BLyS/TNFSF13B Protein

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFF/TNFSF20
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF, also known as BLyS and TALL-1, is a 32 kDa TNF family transmembrane protein that plays a critical role in the development and survival of B lineage cells. BAFF also costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells. It additionally promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages. It can be expressed as a homotrimer or as a heteromer in association with APRIL. BAFF is produced by monocytes, macrophages, neutrophils, dendritic cells, T cells, adipocytes. BAFF signals through the receptors BCMA, TACI, and BAFF R which are primarily expressed by B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF13B
• Uniprot:
  • Q9Y275()
  • Q9Y275()
  • Q9Y275()