Human BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric)

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ELISA: Human BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-50065] - BAFF/BLyS/TNFSF13B ELISA Kit [NBP2-50065] - Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Standard Curve Range
62.5 - 4000 pg/ml
Sensitivity
< 37.5 pg/ml

Order Details

Human BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) Summary

Description
This kit is a sandwich ELISA assay for the quantitative measurement of human BAFF in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of BAFF captured from the samples.
Standard Curve Range
62.5 - 4000 pg/ml
Sensitivity
< 37.5 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
0.1 ml
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFSF13B

Applications/Dilutions

Application Notes
Detection Method: Absorbance (450 nm). Quantitative measurement of BAFF in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Reactivity Notes

No significant cross-reactivity or interference between this analyte and its analogues was observed.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Plate sealers
  2. Lyophilized Standard
  3. Microplate: 96 wells
  4. Stop Solution
  5. SABC dilution buffer
  6. Sample / Standard dilution buffer
  7. TMB substrate
  8. Wash buffer (25X)
  9. HRP-Streptavidin Conjugate (SABC)
  10. Antibody dilution buffer
  11. Biotin-detection antibody (concentrated)

Alternate Names for Human BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric)

  • ApoL related ligand TALL-1
  • B lymphocyte stimulator
  • BAFF
  • BAFFB-cell activating factor
  • B-cell-activating factor
  • BLyS
  • BLYSB-lymphocyte stimulator
  • CD257 antigen
  • CD257
  • Dendritic cell-derived TNF-like molecule
  • DTL
  • TALL1
  • TALL-1delta BAFF
  • TALL1Delta4 BAFF
  • THANK
  • TNF- and APOL-related leukocyte expressed ligand 1
  • TNF and ApoL-related leukocyte expressed ligand 1
  • TNF homolog that activates apoptosis
  • TNFSF13B
  • TNFSF20
  • tumor necrosis factor (ligand) superfamily, member 13b
  • tumor necrosis factor (ligand) superfamily, member 20
  • tumor necrosis factor ligand superfamily member 13B
  • tumor necrosis factor superfamily, member 13b
  • tumor necrosis factor-like protein ZTNF4
  • ZTNF4

Background

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands - 2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNFSF13B