![Western Blot: BAF57 Antibody (JE41-11) [NBP2-76977] - Western blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Western-Blot-NBP2-76977-img0002.jpg "Western Blot: BAF57 Antibody (JE41-11) [NBP2-76977] - Western blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P, IP |
| Clone | JE41-11 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within human BAF57 aa 1-200. |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SMARCE1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
PTMs for BAF57 Antibody (NBP2-76977)Learn more about PTMs related to BAF57 Antibody (NBP2-76977).
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| Gene Symbol | SMARCE1 |
![Immunocytochemistry/Immunofluorescence: BAF57 Antibody (JE41-11) [NBP2-76977] - ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunocytochemistry-Immunofluorescence-NBP2-76977-img0005.jpg "Immunocytochemistry/Immunofluorescence: BAF57 Antibody (JE41-11) [NBP2-76977] - ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunohistochemistry-NBP2-76977-img0009.jpg "Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: BAF57 Antibody (JE41-11) [NBP2-76977] - ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunocytochemistry-Immunofluorescence-NBP2-76977-img0003.jpg "Immunocytochemistry/Immunofluorescence: BAF57 Antibody (JE41-11) [NBP2-76977] - ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: BAF57 Antibody (JE41-11) [NBP2-76977] - ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunocytochemistry-Immunofluorescence-NBP2-76977-img0004.jpg "Immunocytochemistry/Immunofluorescence: BAF57 Antibody (JE41-11) [NBP2-76977] - ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunohistochemistry-NBP2-76977-img0006.jpg "Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunohistochemistry-NBP2-76977-img0007.jpg "Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/BAF57-Antibody-(JE41-11)-Immunohistochemistry-NBP2-76977-img0008.jpg "Immunohistochemistry: BAF57 Antibody (JE41-11) [NBP2-76977] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin.")
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.](http://images.novusbio.com/images2/Brg1-Antibody-Western-Blot-NB100-2594-img0019.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/images2/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0017.jpg)
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/images2/Brg1-Antibody-Western-Blot-NB100-2594-img0018.jpg)
![Western Blot: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis using SMARCA1 mAb against HEK293 (1) and SMARCA1 (AA: 933-1070)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/SMARCA1-Antibody-(2H7B8)-Western-Blot-NBP2-61879-img0006.jpg)
![Immunohistochemistry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of paraffin-embedded rectum cancer tissues using SMARCA1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/SMARCA1-Antibody-(2H7B8)-Immunohistochemistry-NBP2-61879-img0004.jpg)
![Flow Cytometry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of NIH/3T3 cells using SMARCA1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/SMARCA1-Antibody-(2H7B8)-Flow-Cytometry-NBP2-61879-img0002.jpg)
![Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/SMARCC1-Antibody-Western-Blot-NBP2-20415-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCC1 protein stained by SMARCC1 antibody (C2C3), C-term diluted at 1:500. Red-Phalloidin, a cytoskeleton marker- diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/images2/SMARCC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20415-img0025.jpg)
![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded rat hind brain dilution: 1:500.](http://images.novusbio.com/images2/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP1-90015] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/BRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90015-img0007.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-90015-img0011.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.](http://images.novusbio.com/images2/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-90015-img0008.jpg)
![Western Blot: SNF5 Antibody [NBP1-90013] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/SNF5-Antibody-Western-Blot-NBP1-90013-img0010.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/images2/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0013.jpg)
![Western Blot: SNF5 Antibody [NBP1-90013] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/SNF5-Antibody-Western-Blot-NBP1-90013-img0008.jpg)
![Western Blot: SMARCC2/BAF170 Antibody [NBP1-90017] - Analysis using Anti-SMARCC2/BAF170 antibody NBP1-90017 (A) shows similar pattern to a competitors Anti-SMARCC2/BAF170 antibody (B)](http://images.novusbio.com/images2/SMARCC2-BAF170-Antibody-Western-Blot-NBP1-90017-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCC2/BAF170 Antibody [NBP1-90017] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/SMARCC2-BAF170-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90017-img0007.jpg)
![Immunohistochemistry-Paraffin: SMARCC2/BAF170 Antibody [NBP1-90017] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/images2/SMARCC2-BAF170-Antibody-Immunohistochemistry-Paraffin-NBP1-90017-img0002.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/images2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: BANF1 Antibody (3F10-4G12) [H00008815-M01] - BANF1 monoclonal antibody (M01), clone 3F10-4G12 Analysis of BANF1 expression in Jurkat.](http://images.novusbio.com/images2/BANF1 Antibody (3F10-4G12)-Western Blot-H00008815-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: BANF1 Antibody (3F10-4G12) [H00008815-M01] - Analysis of monoclonal antibody to BANF1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/BANF1-Antibody-(3F10-4G12)-Immunohistochemistry-Paraffin-H00008815-M01-img0005.jpg)
![Sandwich ELISA: BANF1 Antibody (3F10-4G12) [H00008815-M01] - Detection limit for recombinant GST tagged BANF1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/BANF1-Antibody-(3F10-4G12)-Sandwich-ELISA-H00008815-M01-img0006.jpg)
![Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Western-Blot-NBP2-38530-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38530-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining in human adrenal gland and lymph node tissues using NBP2-38530 antibody. Corresponding FDXR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-38530-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89146-img0006.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0016.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0010.jpg)
![Western Blot: SMARCD1 Antibody [NBP2-94195] - Analysis of extracts of various cell lines, using SMARCD1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](http://images.novusbio.com/images2/SMARCD1 Antibody-Western Blot-NBP2-94195-img0007.jpg)
![Immunohistochemistry-Paraffin: SMARCD1 Antibody [NBP2-94195] - Paraffin-embedded rat brain using SMARCD1.](http://images.novusbio.com/images2/SMARCD1-Antibody-Immunohistochemistry-Paraffin-NBP2-94195-img0006.jpg)
![Immunohistochemistry-Paraffin: SMARCD1 Antibody [NBP2-94195] - Paraffin-embedded human lung cancer using SMARCD1.](http://images.novusbio.com/images2/SMARCD1-Antibody-Immunohistochemistry-Paraffin-NBP2-94195-img0001.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.](http://images.novusbio.com/images2/SRC1-Antibody-(1135-H4)-Western-Blot-NB200-310-img0003.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: ZNF537 Antibody [NBP2-81880] - Analysis of ZNF537 in mouse brain tissue lysate with ZNF537 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/images2/ZNF537-Antibody-Western-Blot-NBP2-81880-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ZNF537 Antibody [NBP2-81880] - Immunofluorescence of ZNF537 in mouse brain tissue with ZNF537 antibody at 20 ug/mL.](http://images.novusbio.com/images2/ZNF537-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81880-img0003.jpg)
![Immunohistochemistry: ZNF537 Antibody [NBP2-81880] - Immunohistochemistry of ZNF537 in mouse brain tissue with ZNF537 antibody at 2.5 ug/mL.](http://images.novusbio.com/images2/ZNF537-Antibody-Immunohistochemistry-NBP2-81880-img0002.jpg)
![Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2, SK-N-SH, and SJSA-1.](http://images.novusbio.com/images2/Myogenin-Antibody-(5FD)-Western-Blot-NB100-56510-img0010.jpg)
![Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Human myocytes using NB100-56703 at 2.5 ug/mL. Hematoxylin-eosin counterstain.](http://images.novusbio.com/images2/Myogenin-Antibody-(5FD)-Immunohistochemistry-NB100-56510-img0009.jpg)
![Immunohistochemistry-Frozen: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/mL.](http://images.novusbio.com/images2/Myogenin-Antibody-(5FD)-Immunohistochemistry-NB100-56510-img0008.jpg)
![Western Blot: ARID1A Antibody [NBP1-88932] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/images2/ARID1A-Antibody-Western-Blot-NBP1-88932-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ARID1A Antibody [NBP1-88932] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/ARID1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88932-img0012.jpg)
![Immunohistochemistry-Paraffin: ARID1A Antibody [NBP1-88932] - Immunohistochemical staining of human colorectal cancer shows weak to moderate nuclear positivity in tumor cells.](http://images.novusbio.com/images2/ARID1A-Antibody-Immunohistochemistry-Paraffin-NBP1-88932-img0010.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")