BAF53A Antibody

Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: BAF53A Antibody [NB100-1421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: BAF53A Antibody [NB100-1421] - Staining of Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BAF53A Antibody Summary

• Description: Novus Biologicals Goat BAF53A Antibody (NB100-1421) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-BAF53A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence YEEGGKQCVERKCP corresponding to C-Terminus according to NP_004292.1, NP_817126.1, NP_057272.1.
• Epitope: From C terminus.
• Localization: Nuclear
• Specificity: This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Reported variants represent identical protein (NP_817126.1; NP_829888.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACTL6A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:6000
  • Western Blot 0.5 - 2 ug/mL
• Control:

  ACTL6B Overexpression Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BAF53A Antibody

• actin-like 6A
• actin-like protein 6A
• actin-related protein 4,53 kDa BRG1-associated factor A
• Actin-related protein Baf53a
• Actl6
• Arp4
• arpNbeta
• BAF complex 53 kDa subunit
• Baf53a
• BAF53AACTL6
• BAF53MGC5382
• BRG1-associated factor 53A
• hArpN beta
• INO80 complex subunit K
• INO80KARPN-BETA

Background

BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAF53A Antibody (NB100-1421)(1)

Publication using NB100-1421

• Harata M, Mochizuki R, Mizuno S. Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues. Biosci Biotechnol Biochem 1999-05-01 [PMID: 10380635]

Bioinformatics

• Gene Symbol: ACTL6A
• Entrez:
  • 51412(Human)
  • 86(Human)
• Uniprot:
  • NP_057272(Human)
  • O96019(Human)