BAF53A Antibody

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Western Blot: BAF53A Antibody [NB100-1421] - NB100-1421 staining of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BAF53A Antibody Summary

Immunogen
Peptide with sequence YEEGGKQCVERKCP corresponding to C-Terminus according to NP_004292.1, NP_817126.1, NP_057272.1.
Epitope
From C terminus.
Localization
Nuclear
Specificity
This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Reported variants represent identical protein (NP_817126.1; NP_829888.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACTL6A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 48 kDa band observed in Hela lysates (predicted MW of 49 kDa band according to NP_057272, NP_004292).
Positive Control
ACTL6B Lysate (NBL1-07278)
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-1421.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAF53A Antibody

  • actin-like 6A
  • actin-like protein 6A
  • actin-related protein 4,53 kDa BRG1-associated factor A
  • Actin-related protein Baf53a
  • Actl6
  • Arp4
  • arpNbeta
  • BAF complex 53 kDa subunit
  • Baf53a
  • BAF53AACTL6
  • BAF53MGC5382
  • BRG1-associated factor 53A
  • hArpN beta
  • INO80 complex subunit K
  • INO80KARPN-BETA

Background

BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAF53A Antibody (NB100-1421)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACTL6A
Entrez
Uniprot