Human BACH1 ELISA Kit (Colorimetric)

ELISA: Human BACH1 ELISA Kit (Colorimetric) [NBP3-18220] - Standard Curve Reference

Product Discontinued

Human BACH1 ELISA Kit (Colorimetric) Summary

• Description: The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human BACH1 in the sample, this Human BACH1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human BACH1 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Standard Curve Range: 46.87-3000 pg/ml
• Sensitivity: 22.15 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 10
• Spike Recovery: 81-113%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BACH1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human BACH1 ELISA Kit (Colorimetric)

• BACH1
• basic region leucine zipper transcriptional regulator BACH1
• BTB and CNC homolog 1
• BTB and CNC homology 1, basic leucine zipper transcription factor 1
• BTB
• HA2303
• transcription regulator protein BACH1
• transcription regulator protein10BACH-1

Background

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for BACH1 ELISA Kit (NBP3-18220). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BACH1