BACH1 Antibody

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5777) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BACH1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse BACH1
  Ser133-Gly513
  Accession # P97302
• Specificity: Detects endogenous mouse BACH1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BACH1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BACH1 Antibody

• BACH1
• basic region leucine zipper transcriptional regulator BACH1
• BTB and CNC homolog 1
• BTB and CNC homology 1, basic leucine zipper transcription factor 1
• BTB
• HA2303
• transcription regulator protein BACH1
• transcription regulator protein10BACH-1

Background

BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Mouse BACH1 is 739 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 565‑580), and a Leu-zipper domain (aa 588‑610). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. Over aa 133‑513, mouse BACH1 shares 75% and 88% aa identity with human and rat BACH1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BACH1 Antibody (AF5777)(5)

Publications using AF5777

• A Galmozzi, BP Kok, AS Kim, JR Montenegro, JY Lee, R Spreafico, S Mosure, V Albert, R Cintron-Co, C Godio, WR Webb, B Conti, LA Solt, D Kojetin, CG Parker, JJ Peluso, JK Pru, G Siuzdak, BF Cravatt, E Saez PGRMC2 is an intracellular haem chaperone critical for adipocyte function Nature, 2019-11-20;0(0):. 2019-11-20 [PMID: 31748741] (Western Blot, Mouse)
• Bennett L F, Liao C et al. Inflammation induces stress erythropoiesis through heme-dependent activation of SPI-C. Sci Signal 10/9/2019 [PMID: 31506384]
• Pei Jin Lim, Tiago L. Duarte, João Arezes, Daniel Garcia-Santos, Amel Hamdi, Sant-Rayn Pasricha et al. Nrf2 controls iron homoeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin Nature Metabolism 7/6/2019 [PMID: 31276102]
• Luca Lignitto, Sarah E. LeBoeuf, Harrison Homer, Shaowen Jiang, Manor Askenazi, Triantafyllia R. Karakousi et al. Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1 Cell 7/1/2019 [PMID: 31257023]
• A Wang, SD Pope, JS Weinstein, S Yu, C Zhang, CJ Booth, R Medzhitov Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice Proc. Natl. Acad. Sci. U.S.A., 2019-01-23;0(0):. 2019-01-23 [PMID: 30674681] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: BACH1
• Uniprot:
  • P97302()