BACE-2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

BACE-2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human BACE-2.
  
  

  Source: E. coli

  Amino Acid Sequence: FRCQRRPRDPEVVNDESSLVRHRWK

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: BACE2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10-100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-68624.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for BACE-2 Recombinant Protein Antigen

• AEPLC
• Asp 1
• ASP1
• ASP21
• Aspartic-like protease 56 kDa
• Aspartyl protease 1
• BACE2
• BACE-2
• beta secretase 2
• beta-secretase 2
• beta-site amyloid beta A4 precursor protein-cleaving enzyme 2
• Beta-site amyloid precursor protein cleaving enzyme 2
• Beta-site APP cleaving enzyme 2
• beta-site APP-cleaving enzyme 2
• beta-site APP-cleaving enzyme 2, EC 3.4.2310ALP5656 kDa aspartic-like protease
• CEAP1
• Down region aspartic protease
• Down syndrome region aspartic protease
• DRAPBAE2
• EC 3.4.23
• EC 3.4.23.45
• memapsin-1
• Membrane-associated aspartic protease 1
• theta-secretase
• transmembrane aspartic proteinase Asp1

Background

BACE2 (Memapsin 1) is a membrane-bound aspartic protease that is highly homologous with BACE1 (Memapsin 2). While BACE1 processes the amyloid precursor protein (APP) at a key step in generating the beta-amyloid peptide and presumably causes Alzheimer's disease (AD), BACE2 has not been demonstrated to be directly involved in APP processing, and its physiological functions remain to be determined. In vivo, BACE2 is expressed as a precursor protein containing pre-, pro-, protease, transmembrane, and cytosolic domains/peptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BACE2