BACE-2 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

BACE-2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BACE-2 Ectodomain
  Gly63-Pro466
  Accession # Q9Y5Z0
• Specificity: Detects human and mouse BACE‑2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) BACE‑1, rhCathepsin D, and rhCathepsin E is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BACE-2 Antibody [Alexa Fluor® 350]

• AEPLC
• Asp 1
• ASP1
• ASP21
• Aspartic-like protease 56 kDa
• Aspartyl protease 1
• BACE2
• BACE-2
• beta secretase 2
• beta-secretase 2
• beta-site amyloid beta A4 precursor protein-cleaving enzyme 2
• Beta-site amyloid precursor protein cleaving enzyme 2
• Beta-site APP cleaving enzyme 2
• beta-site APP-cleaving enzyme 2
• beta-site APP-cleaving enzyme 2, EC 3.4.2310ALP5656 kDa aspartic-like protease
• CEAP1
• Down region aspartic protease
• Down syndrome region aspartic protease
• DRAPBAE2
• EC 3.4.23
• EC 3.4.23.45
• memapsin-1
• Membrane-associated aspartic protease 1
• theta-secretase
• transmembrane aspartic proteinase Asp1

Background

BACE-2 ( beta -site APP cleaving enzyme 2; also ASP-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both inside and outside the CNS and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear. If it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa’s 329‑378, while a second shows an 18 aa substitution for aa’s 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE‑2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.