BAAT1 Antibody

Images

 
Western Blot: BAAT1 Antibody [NB100-2256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Rabbit anti- BAAT1 antibody NB100-2256 used for WB at 0.04 ...read more
Immunohistochemistry: BAAT1 Antibody [NB100-2256] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAAT1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using ...read more
Immunohistochemistry-Paraffin: BAAT1 Antibody [NB100-2256] - FFPE section of human metastatic lymph node. NB100-2256 used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

BAAT1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 775 and the C-terminus (residue 821) of human BRCA1-associated protein required for ATM activation-1 (C7orf27) using the numbering given in entry NP_689956.1 (GeneID 221927).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRAT1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAAT1 Antibody

  • BAAT1
  • BRCA1-associated ATM activator 1
  • BRCA1-associated protein required for ATM activation protein 1
  • C7orf27
  • chromosome 7 open reading frame 27
  • HEAT repeat-containing protein C7orf27
  • MGC22916

Background

BAAT1 (BRCA1-associated protein required for ATM activation-1) was identified by yeast two hybrid analysis. BAAT1 binds to ATM, localizes to double-strand breaks, and is required for Ser1981 phosphorylation of ATM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BRAT1
Entrez
Uniprot