![Western Blot: BAAT1 Antibody (JE64-71) [NBP3-32089] - Western blot analysis of BAAT1 on different lysates with Rabbit anti-BAAT1 antibody (NBP3-32089) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-3 cell lysate Lane 3: 293 cell lysate Lane 4: K562 cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32089) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32089_rabbit-baat1-mab-je64-71-278202415295594.jpg "Western Blot: BAAT1 Antibody (JE64-71) [NBP3-32089] - Western blot analysis of BAAT1 on different lysates with Rabbit anti-BAAT1 antibody (NBP3-32089) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-3 cell lysate Lane 3: 293 cell lysate Lane 4: K562 cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32089) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | JE64-71 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human BAAT1 aa 672-821/821. (Uniprot: Q6PJG6) |
| Localization | Cytoplasm, Nucleus. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BRAT1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 88 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BAAT1 Antibody (NBP3-32089)Find related products by research area.
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| Gene Symbol | BRAT1 |
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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