B7-H4 Antibody (H74) [PE]

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Flow Cytometry: B7-H4 Antibody (H74) [PE] [NBP1-43743] - See notes for image caption

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
H74
Clonality
Monoclonal
Host
Mouse
Conjugate
PE
Concentration
0.2 mg/ml

Order Details

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B7-H4 Antibody (H74) [PE] Summary

Immunogen
The immunogen for this antibody was Purified B7H4.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VTCN1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for B7-H4 Antibody (H74) [PE]

  • B7h.5
  • B7H4
  • B7-H4
  • B7H4T-cell costimulatory molecule B7x
  • B7S1
  • B7S1VCTN1
  • B7x
  • B7XPRO1291
  • FLJ22418
  • Immune costimulatory protein B7-H4
  • Protein B7S1
  • T cell costimulatory molecule B7x
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain-containing T-cell activation inhibitor 1
  • Vtcn1

Background

The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x. Cross reactivity of this antibody to other proteins has not been determined. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand. H74 stains human B7-H4 transfected cells and not peripheral blood cells. Exact expression pattern of B7-H4 has not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VTCN1
Entrez