B7-H4 Antibody (H74)


Flow Cytometry: B7-H4 Antibody (H74) [NBP1-43273] - Analysis using the Biotin conjugate of NBP1-43273. Staining of non-transfected (left) and human B7-H4 transfected (right) 293T cells with Mouse IgG1 kappa Isotype ...read more
Flow Cytometry: B7-H4 Antibody (H74) [NBP1-43273] - Staining of non-transfected (left) and human B7-H4-transfected (right) HEK 293T cells with 0.125 ug of Mouse IgG1 kappa Isotype Control, Purified (NBP1-43319) (open ...read more
Flow Cytometry: B7-H4 Antibody (H74) [NBP1-43273] - Analysis using the PE conjugate of NBP1-43273. Analysis of transient hB7-H4 transfected cells using B7H4 PE antibody.

Product Details

Reactivity HuSpecies Glossary
Applications Flow
0.5 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

B7-H4 Antibody (H74) Summary

The immunogen for this antibody was Purified B7H4.
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry < = 0.25 ug/10^6 cells in 100 ul
Application Notes
This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Alternate Names for B7-H4 Antibody (H74)

  • B7h.5
  • B7H4
  • B7-H4
  • B7H4T-cell costimulatory molecule B7x
  • B7S1
  • B7S1VCTN1
  • B7x
  • B7XPRO1291
  • FLJ22418
  • Immune costimulatory protein B7-H4
  • Protein B7S1
  • T cell costimulatory molecule B7x
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain-containing T-cell activation inhibitor 1
  • Vtcn1


The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x. Cross reactivity of this antibody to other proteins has not been determined. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand. H74 stains human B7-H4 transfected cells and not peripheral blood cells. Exact expression pattern of B7-H4 has not been fully characterized.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-43517
PE Labeled NBP1-43743

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Gene Symbol VTCN1

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