B7-H4 Antibody (H74)

Flow Cytometry: B7-H4 Antibody (H74) [NBP1-43273] - Analysis using the PE conjugate of NBP1-43273. Analysis of transient hB7-H4 transfected cells using B7H4 PE antibody.

Flow Cytometry: B7-H4 Antibody (H74) [NBP1-43273] - Staining of non-transfected (left) and human B7-H4-transfected (right) HEK 293T cells with 0.125 ug of Mouse IgG1 kappa Isotype Control, Purified (NBP1-43319) (open histogram) or 0.125 ug of Anti-Human B7H4 Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE (NBP1-97922). Total cells were used for analysis.

Flow Cytometry: B7-H4 Antibody (H74) [NBP1-43273] - Analysis using the Biotin conjugate of NBP1-43273. Staining of non-transfected (left) and human B7-H4 transfected (right) 293T cells with Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.06 ug Anti-Human B7H4 Biotin (filled histogram) followed by Streptavidin PE. Total cells were used for analysis.

Product Discontinued

B7-H4 Antibody (H74) Summary

• Immunogen: The immunogen for this antibody was Purified B7H4.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VTCN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.25 ug/10^6 cells in 100 ul
• Application Notes: This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for B7-H4 Antibody (H74)

• B7h.5
• B7H4
• B7-H4
• B7H4T-cell costimulatory molecule B7x
• B7S1
• B7S1VCTN1
• B7x
• B7XPRO1291
• FLJ22418
• Immune costimulatory protein B7-H4
• Protein B7S1
• T cell costimulatory molecule B7x
• V-set domain containing T cell activation inhibitor 1
• V-set domain-containing T-cell activation inhibitor 1
• Vtcn1

Background

The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x. Cross reactivity of this antibody to other proteins has not been determined. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand. H74 stains human B7-H4 transfected cells and not peripheral blood cells. Exact expression pattern of B7-H4 has not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VTCN1
• Entrez:
  • 79679(Human)
• Uniprot:
  • Q7Z7D3()