B7-H4 Antibody (7A1-6) - Chimeric

Immunocytochemistry/Immunofluorescence: B7-H4 Antibody (7A1-6) - Chimeric [NBP3-09034] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 7A1-6 (NBP3-09034) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09034, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

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B7-H4 Antibody (7A1-6) - Chimeric Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising rats with the recombinant murine B7-H4.
• Specificity: This antibody is specific for murine B7-H4, a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VTCN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Functional Assay
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Purity: Protein A purified

Alternate Names for B7-H4 Antibody (7A1-6) - Chimeric

• B7h.5
• B7H4
• B7-H4
• B7H4T-cell costimulatory molecule B7x
• B7S1
• B7S1VCTN1
• B7x
• B7XPRO1291
• FLJ22418
• Immune costimulatory protein B7-H4
• Protein B7S1
• T cell costimulatory molecule B7x
• V-set domain containing T cell activation inhibitor 1
• V-set domain-containing T-cell activation inhibitor 1
• Vtcn1

Background

B7H4 is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected, thus suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC). There are three different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VTCN1