B4GALNT1 Antibody (5F9) - Azide and BSA Free

Western Blot: B4GALNT1 Antibody (5F9) [H00002583-M02] - B4GALNT1 monoclonal antibody (M02), clone 5F9. Analysis of B4GALNT1 expression in PC-12.

Western Blot: B4GALNT1 Antibody (5F9) [H00002583-M02] - B4GALNT1 monoclonal antibody (M02), clone 5F9 Analysis of B4GALNT1 expression in HeLa.

Sandwich ELISA: B4GALNT1 Antibody (5F9) [H00002583-M02] - Detection limit for recombinant GST tagged B4GALNT1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 5F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

B4GALNT1 Antibody (5F9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: B4GALNT1 (NP_001469, 30 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. APGLRLPLAPWAPPQSPRRPELPDLAPEPRYAHIPVRIKEQVVGLLAWNNCSCESSGGGLPLPFQKQVRAIDLTKAFDPAELRAASATREQEFQAFLS
• Specificity: B4GALNT1 - beta-1,4-N-acetyl-galactosaminyl transferase 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B4GALNT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for B4GALNT1 Antibody (5F9) - Azide and BSA Free

• (N-acetylneuraminyl)-galactosylglucosylceramide
• B4GALNT1
• beta-1,4 N-acetylgalactosaminyltransferase 1
• beta1,4GalNAc-T
• beta-1,4-N-acetyl-galactosaminyl transferase 1
• beta1-4GalNAc-T
• GALGT
• GALGTEC 2.4.1.92
• GALNACT
• GalNAc-T
• GD2 Synthase
• GD2 synthase, GM2 synthase
• GM2 Synthase
• GM2/GD2 synthase
• SIAT2
• SPG26
• UDP-Gal:betaGlcNAc beta-1,4-N-acetylgalactosaminyltransferase transferase 1
• UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramideN-acetylgalactosaminyltransferase (GalNAc-T)

Background

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B4GALNT1
• Entrez:
  • 2583(Human)
• OMIM:
  • 601873(Human)
• Uniprot:
  • NP_001469(Human)