B3GNT5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: B3GNT5 Antibody [NBP1-88954] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.

Immunohistochemistry-Paraffin: B3GNT5 Antibody [NBP1-88954] - Staining of human liver shows moderate granular positivity in hepatocytes.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

B3GNT5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit B3GNT5 Antibody - BSA Free (NBP1-88954) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B3GNT5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  B3GNT5 Protein (NBP1-88954PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for B3GNT5 Antibody - BSA Free

• B3GN-T5
• beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase
• beta-1,3-Gn-T5
• Beta-1,3-N-acetylglucosaminyltransferase 5
• beta-1,3-N-acetylglucosaminyltransferase bGnT-5
• beta3Gn-T5
• BGnT-5
• EC 2.4.1
• EC 2.4.1.-
• Lactotriaosylceramide synthase
• Lc(3)Cer synthase
• Lc3 synthase
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5

Background

The B3GNT5 gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membraneprotein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likelycandidate for lactotri

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B3GNT5