B-Raf Antibody (3D2)

Western Blot: B-Raf Antibody (3D2) [H00000673-M02] - BRAF monoclonal antibody (M02), clone 3D2. Analysis of BRAF expression in human pancreas.

Proximity Ligation Assay: B-Raf Antibody (3D2) [H00000673-M02] - Analysis of protein-protein interactions between MAPK3 and BRAF. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: B-Raf Antibody (3D2) [H00000673-M02] - Detection limit for recombinant GST tagged BRAF is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

B-Raf Antibody (3D2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: BRAF (NP_004324, 346 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRD
• Specificity: v-raf murine sarcoma viral oncogene homolog B1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BRAF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for B-Raf Antibody (3D2)

• 94 kDa B-raf protein
• B-Raf proto-oncogene serine/threonine-protein kinase (p94)
• BRaf
• B-Raf
• BRAF1
• B-RAF1
• BRAF1MGC126806
• EC 2.7.11.1
• MGC138284
• murine sarcoma viral (v-raf) oncogene homolog B1
• NS7
• p94
• Proto-oncogene B-Raf
• RAFB1
• serine/threonine-protein kinase B-raf
• v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B-Raf Antibody (H00000673-M02)(2)

Publications using H00000673-M02

• Shaw J, Dale I, Hemsley P et al. Positioning High-Throughput CETSA in Early Drug Discovery through Screening against B-Raf and PARP1. SLAS Discov. 2019-01-03 [PMID: 30543471]
• Liu CH, Chen TC, Chau GY et al. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Mol Cell Proteomics 2013-02-08 [PMID: 23397142]

Bioinformatics

• Gene Symbol: BRAF
• Entrez:
  • 673(Human)
• OMIM:
  • 164757(Human)
• Uniprot:
  • NP_004324(Human)