AZ1 Antibody

Western Blot: AZ1 Antibody [NB100-68227] - Detection of Human CEP131/AZ1 on HeLa whole cell lysate using NB100-68227. CEP131/AZ1 was also immunoprecipitated by rabbit anti-CEP131/AZ1 antibodies NB100-68225 and NB100-68226.

Immunocytochemistry/Immunofluorescence: AZ1 Antibody [NB100-68227] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:200.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

AZ1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1033 and 1083 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEP131
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: ICC/IF Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AZ1 Antibody

• 5-azacytidine induced 1
• 5-azacytidine-induced protein 1
• AZ1
• centrosomal protein 131 kDa
• centrosomal protein of 131 kDa
• CEP131
• KIAA1118
• pre-acrosome localization protein 1
• ZA1

Background

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEP131
• Entrez:
  • 22994(Human)
• Uniprot:
  • EAW89632.1(Human)